Anti-RRAGC Antibody (CAB7479)

Antibody Name:	Anti-RRAGC Antibody
Antibody SKU:	CAB7479
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human RRAGC (NP_071440.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, 293T, HL-60, Mouse lung, Mouse ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human RRAGC (NP_071440.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSLQ YGAE ETPL AGSY GAAD SFPK DFGY GVEE EEEE AAAA GGGV GAGA GGGC GPGG ADSS KPRI LLMG LRRS GKSS IQKV VFHK MSPN ETLF LEST NKIY KDDI SNSS FVNF QIWD FPGQ MDFF DPTF DYEM IFRG TGAL IYVI DAQD DYME ALTR LHIT VSKA YKVN PDMN FEVF IHKV DGLS DDHK IETQ RDIH QRAN DDLA DAGL EKLH LSFY LTSI YDHS IFEA FSKV VQKL IPQL PTLE NLLN IF
Gene ID:	64121
Uniprot:	Q9HB90
Cellular Location:	Cytoplasm, Lysosome, Nucleus
Calculated MW:	44kDa
Observed MW:	44kDa

Synonyms:	RRAGC, GTR2, RAGC, TIB929
Background:	This gene encodes a member of the GTR/RAG GTP-binding protein family. The encoded protein is a monomeric guanine nucleotide-binding protein which forms a heterodimer with RRAGA and RRAGB and is primarily localized to the cytoplasm. The encoded protein promotes intracellular localization of the mTOR complex. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	RRAGC: Has guanine nucleotide-binding activity but weak intrinsic GTPase activity. Probably required for the amino acid- induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Forms a heterodimer with RRAGA in a sequence-independent manner. Binds GTP. Interacts with NOL8 and RRAGB. Belongs to the GTR/RAG GTP-binding protein family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 1p34 Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; lysosome; nucleus Molecular Function:GDP binding; GTP binding; GTPase activity; magnesium ion binding; protein binding; protein heterodimerization activity Biological Process: apoptosis; cell cycle arrest; cell growth; cellular response to amino acid starvation; gene expression; insulin receptor signaling pathway; macroautophagy; regulation of TOR signaling pathway; RNA splicing; small GTPase mediated signal transduction; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent
NCBI Summary:	This gene encodes a member of the GTR/RAG GTP-binding protein family. The encoded protein is a monomeric guanine nucleotide-binding protein which forms a heterodimer with RRAGA and RRAGB and is primarily localized to the cytoplasm. The encoded protein promotes intracellular localization of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]
UniProt Code:	Q9HB90
NCBI GenInfo Identifier:	74752776
NCBI Gene ID:	64121
NCBI Accession:	Q9HB90.1
UniProt Secondary Accession:	Q9HB90,Q9H202, Q9H8Q8,
UniProt Related Accession:	Q9HB90
Molecular Weight:	44,224 Da
NCBI Full Name:	Ras-related GTP-binding protein C
NCBI Synonym Full Names:	Ras related GTP binding C
NCBI Official Symbol:	RRAGC
NCBI Official Synonym Symbols:	GTR2; RAGC; TIB929
NCBI Protein Information:	ras-related GTP-binding protein C
UniProt Protein Name:	Ras-related GTP-binding protein C
UniProt Synonym Protein Names:	GTPase-interacting protein 2; TIB929
Protein Family:	Ras-related GTP-binding protein
UniProt Gene Name:	RRAGC
UniProt Entry Name:	RRAGC_HUMAN

	Western blot analysis of extracts of various cell lines, using RRAGC antibody (CAB7479) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat intestine using RRAGC Antibody (CAB7479) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using RRAGC Antibody (CAB7479) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse intestine using RRAGC Antibody (CAB7479) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using RRAGC antibody (CAB7479).

Antibodies

RRAGC Antibody (PACO01403)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)