15-PGDH Activity Assay Kit (Fluorometric) (BN00792)

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BN00792
  • 15-PGDH Activity Assay Kit (Fluorometric) (BN00792)
  • 15-PGDH Activity Assay Kit (Fluorometric) (BN00792)
  • 15-PGDH Activity Assay Kit (Fluorometric) (BN00792)
€613

Description

ELISA Kit Technical ManualMSDS

15-PGDH Activity Assay Kit (Fluorometric)

15-Hydroxy prostaglandin dehydrogenase (15-PGDH; EC:1.1.1.141) is a rate-limiting enzyme for prostaglandin catabolism. It oxidizes prostaglandins (PG) to 15-keto metabolites. In general, 15-keto metabolites show reduced biological activity, when compared to PG. PGs are generated from arachidonic acid by cyclooxygenase (COX), and play an important role in inflammatory responses. Recent study found that 15-PGDH serves as a degrading enzyme for PG and serves as a tumor suppressor to inhibit cancer progression. Thus, study of 15-PGDH activity can elucidate and clarify unknown aspects related to cancer development and therapeutics. In Assay Genie's 15-PGDH Assay Kit, 15-PGDH oxidizes a substrate forming intermediates and NADH. The oxidation of NADH reduces a probe generating fluorescence at Ex/Em=535/587 nm. The activity of 15-PGDH is proportional to the fluorescent signal. Assay Genie's 15-PGDH Activity Assay Kit offers a rapid, simple, sensitive, and reliable test suitable for detecting as low as 1 µU of 15-PGDH activity

Figure: (a) NADH Standard Curve. (b) Kinetic measurement of 15-PGDH Specific Activity in lysates prepared from mouse liver (50 µg) and 15-PGDH Positive Control. (c) 15-PGDH specific activity of mouse lysates from liver (50 µg), kidney (16.5 µg) and lung (41 µg). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00792

Size

100 assays

Kit Summary

  • Detection method- Fluorescence (Ex/Em 535/587 nm)
  • Species reactivity- Mammalian
  • Application- The 15-PGDH fluorometric assay kit is a highly sensitive assay and can detect < 5 µU 12-PGDH activity (based on our unit definition) in a variety of samples.

Detection Method

Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Eukaryotes

Applications

  • Measurement of 15-PGDH activity in various tissues/cells.
  • Analysis of prostaglandin catabolism in various cell types.

Features and Benefits

  • Simple procedure
  • Fast and convenient
  • Sensitive assay for measuring 15-PGDH activity in various biological samples and the kit is also suitable for high throughput analyses.

Kit Components

  • 15-PGDH Assay Buffer (in DMSO)
  • 15-PGDH Developer
  • ALDH Substrate (in DMSO)
  • NADH Standard
  • 15-PGDH Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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