2-NBDG Glucose Uptake Assay Kit (Cell-Based) (BN00906)

(No reviews yet) Write a Review
SKU:
BN00906
€607

Description

ELISA Kit Technical ManualMSDS

2-NBDG Glucose Uptake Assay Kit (Cell-Based)

Glucose is a ubiquitous energy source in most organisms and plays a pivotal role in cellular metabolisms and homeostasis. Cancer cells exhibit increased glucose uptake to support their high proliferation rate. 2-NBDG (2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose) is a fluorescent deoxyglucose analog that can be taken up by cells through glucose transporters. However, 2-NBDG cannot be fully utilized in glycolysis because of its modification and thus accumulates inside the cells. Fluorescence generated by this fluorescent glucose analog is proportional to glucose uptake by the cells and can be used to measure glucose uptake using fluorescent microscopy and flow cytometry. To validate the assay, the kit includes phloretin, a natural phenol that inhibits glucose uptake. This easy to use non-radioactive kit allows imaging and accurate measurement of glucose uptake in cultured cells in response to insulin, growth factors etc.

Figure: Glucose uptake in Jurkat and HeLa cells. 2.5x105 Jurkat cells were pretreated with or without 4 µl phloretin (1X concentration) for 45 min. After pretreatment, cells were washed and incubated with 2-NBDG Reagent, Glucose Uptake Enhancer, and the same concentration of phloretin for another 30 min. according to kit’s protocol. (A) Comparison of histograms from flow analysis showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin)

Key Information Description


Product SKU

BN00906

Size

50 assays

Detection Method

FACS (488 nm excitation laser) and fluorescent microscope (excitation range 420 nm-495 nm).

Applications

  • Measurement of glucose uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc.
  • Dual-staining of glucose transporters and glucose uptake
  • Analysis of glucose metabolism and cell signaling in various cell types
  • Screening of anti-diabetic compounds

Features & Benefits

  • Easy-to-use
  • Non-radioactive
  • Image and accurately measure glucose uptake in cultured cells in response to insulin, growth factors etc.

Kit Components

  • Analysis Buffer (50X)
  • 2-NBDG Reagent (100X)
  • Glucose Uptake Enhancer
  • Phloretin (100X)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose