5-Lipoxygenase Inhibitor Screening Kit (Fluorometric) (BN01138)

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ELISA Kit Technical ManualMSDS

5-Lipoxygenase Inhibitor Screening Kit (Fluorometric)

5-Lipoxygenase (5-LOX) is a non-heme iron-containing dioxygenase that converts unsaturated fatty acids to epoxides; for example the synthesis of leukotrienes from arachidonic acid. It is involved in processes like cell proliferation, differentiation and inflammation and has been implicated in inflammation and hyper proliferation mediated diseases like asthma, rheumatoid arthritis and cancer. Research for identifying potential remedies for such diseases involves the identification of inhibitors of 5-lipoxygenase. Assay Genie's 5-Lipoxygenase Inhibitor Screening Kit uses Zileuton, a well-known 5-lipoxygenase inhibitor as positive control, and provides a simple and fast method to screen/ characterize potential 5-lipoxygenase inhibitors

Figure: Inhibition of 5-Lipoxygenase enzyme activity by Zileuton. IC50 of zileuton was determined to be 0.53 ± 0.04 µM. Assays were performed using kit protocol.

Key Info Description

Product SKU



100 assays

Detection Method

Fluorescence (Ex/Em 500/536 nm)

Species Reactivity



Screening of potential inhibitors of 5-lipoxygenase

Features & Benefits

  • Simple procedure; takes only ~40 min
  • Fast and convenient

Kit Components

  • LOX Assay Buffer
  • LOX Probe
  • LOX Substrate
  • Zileuton
  • 5-LOX Enzyme

Storage Conditions


Shipping Conditions

Dry Ice


For Research Use Only! Not For Use in Humans

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Additional Information

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