6-Phosphogluconate Dehydrogenase Activity Colorimetric Assay Kit (BN00769)

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BN00769
  • 6-Phosphogluconate Dehydrogenase Activity Colorimetric Assay Kit (BN00769)
  • 6-Phosphogluconate Dehydrogenase Activity Colorimetric Assay Kit (BN00769)
  • 6-Phosphogluconate Dehydrogenase Activity Colorimetric Assay Kit (BN00769)
€702

Description

ELISA Kit Technical ManualMSDS

6-Phosphogluconate Dehydrogenase Activity Colorimetric Assay Kit

6-Phosphogluconate dehydrogenase (6-PGD, EC 1.1.1.44) is an enzyme in the pentose phosphate pathway that catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose-5-phosphate with simultaneous reduction of NADP+ to NADPH. Ribulose-5-phosphate is used for production of nucleotides and nucleic acids and can also be reversibly converted to glyceraldehyde-3-phosphate and fructose-6-phosphate which can be used in the glycolysis pathway. It also has a vital role in production of NADPH, which is utilized as a reducing agent in numerous biosynthetic pathways and is important in prevention of oxidative damage. In humans, 6-PGD deficiency is an autosomal hereditary disorder that can result in depletion of NADPH in red blood cells. As NADPH is required for regeneration of the cellular antioxidant glutathione in red blood cells, 6-PGD deficiency increases the risk of hemolytic anemia in situations of oxidative stress. On the other hand, inhibition of 6-PGD has been linked to anti-cancer activity by decreasing RNA biosynthesis and increasing the buildup of reactive oxygen species in tumor cells. Assay Genie's 6-PGD assay kit provides a quick, sensitive and easy way for measuring 6-PGD activity in various biological samples. In this assay, 6-PGD converts 6-phosphogluconate into an intermediate, generating NADPH, which subsequently reduces a colorless probe into a strongly colored product detectable by absorbance at 460 nm. The assay is high-throughput adaptable and can detect less than 0.05 mU of 6-PGD activity

Figure: (a) NADPH standard curve; (b) Reaction kinetics of 6-Phosphogluconate dehydrogenase activity in positive control, rat liver (66 µg protein) and rat spleen (56 µg protein) using appropriate background controls; (c) 6-Phosphogluconate dehydrogenase specific activity calculated in rat liver and spleen tissue lysates. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00769

Size

100 assays

Detection Methods

Colorimetric (OD 460 nm)

Species Reactivity

Mammalian

Applications

Measurement of 6-PGD activity in purified/crude enzyme preparations and biological samples.

Features and Benefits

  • Simple, rapid & convenient assay to measure 6-PGD
  • Activity in biological samples
  • Includes Positive Control

Kit Components

  • 6-PGD Assay Buffer
  • 6-PGD Substrate
  • 6-PGD Developer
  • NADH Standard
  • 6-PGD Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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