6-Phosphogluconic Acid (6-PGA) Assay Kit (Colorimetric) (BN00494)

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BN00494
  • 6-Phosphogluconic Acid (6-PGA) Assay Kit (Colorimetric) (BN00494)
  • 6-Phosphogluconic Acid (6-PGA) Assay Kit (Colorimetric) (BN00494)
€732

Description

ELISA Kit Technical ManualMSDS

6-Phosphogluconic Acid (6-PGA) Assay Kit (Colorimetric)

6-Phosphogluconate (6-PGA) is an intermediate of both Pentose Phosphate Pathway (PPP) and Entner-Doudoroff Pathway. It is produced by the hydrolysis of 6-Phosphogluconolactone, catalyzed by 6-Phosphogluconolactonase. In the Pentose Phosphate Pathway, 6-PGA is utilized by 6-Phosphogluconate Dehydrogenase to generate ribulose-5-Phosphate and NADPH. These products are important for nucleic acid synthesis and various anabolic processes. In Prokaryotes, 6-Phosphogluconate is the main metabolite of Entner-Doudoroff pathway, and is converted into Pyruvate using both 6-Phosphogluconate Dehydratase and 2-Keto-3-Deoxyphosphogluconate aldolase. Recent studies show that long-term exposure to glucose perturbs the Pentose Phosphate Pathway, causes significant accumulation of 6-Phosphogluconate and impairs beta cell function. Measurement of 6-Phosphogluconate levels therefore is important for evaluating Pentose Phosphate Pathway, developing therapeutic approaches for diabetes research, and analyzing the Entner-Doudoroff Pathway in bacteria. Assay Genie's 6-Phosphogluconate assay kit can be used with a variety of sample types. In this assay, 6-Phosphogluconate is converted to Ribulose-5-Phosphate by 6-Phosphogluconate Dehydrogenase in the presence of NAD, to form NADH, which reduces a probe and generates strong absorbance at 450 nm. This 6-Phosphogluconate Assay Kit is simple, sensitive & easy to use and can detect 6-Phosphogluconate levels lower than 20 µM.

Figure: (a).6-Phosphogluconate standard curve, n=3 (b). Measurement of 6-Phosphogluconate in the lysates from Rat Liver (160 µg), Rat Kidney (120 µg), and Rat Heart (60 µg). Assays were performed following kit protocol.

Key Information Description

Product SKU

BN00494

Size

100 assays

Detection Methods

Absorbance (OD= 450 nm).

Species Reactivity

Mammalian

Applications

  • Measurement of 6-Phosphogluconic Acid in various tissues/cells.
  • Analysis of Pentose Phosphate Pathway and Entner-Doudoroff Pathway.

Features and Benefits

  • Simple, fast and convenient assay
  • Can measure 6-PGA levels as low as 20 µM

Kit Components

  • 6-PGA Assay Buffer
  • 6-PGA Enzyme
  • 6-PGA Substrate Mix
  • 6-PGA Standard

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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