Anti-ABAT Antibody (CAB5299)

Antibody Name:	Anti-ABAT Antibody
Antibody SKU:	CAB5299
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-300 of human ABAT (NP_001120920.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Mouse brain, Mouse kidney, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-300 of human ABAT (NP_001120920.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SQAA AKVD VEFD YDGP LMKT EVPG PRSQ ELMK QLNI IQNA EAVH FFCN YEES RGNY LVDV DGNR MLDL YSQI SSVP IGYS HPAL LKLI QQPQ NASM FVNR PALG ILPP ENFV EKLR QSLL SVAP KGMS QLIT MACG SCSN ENAL KTIF MWYR SKER GQRG FSQE ELET CMIN QAPG CPDY SILS FMGA FHGR TMGC LATT HSKA IHKI DIPS FDWP IAPF PRLK YPLE EFVK ENQQ EEAR CLEE VEDL IVKY RKKK KTVA GIIV EPIQ SEGG
Gene ID:	18
Uniprot:	P80404
Cellular Location:	Mitochondrion matrix
Calculated MW:	56kDa
Observed MW:	50KDa

Synonyms:

ABAT, GABA-AT, GABAT, NPD009

Background:

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.

UniProt Protein Function:	ABAT: Catalyzes the conversion of gamma-aminobutyrate and L- beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine. Defects in ABAT are a cause of GABA transaminase deficiency (GABATD). The phenotype of this deficiency includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Carbohydrate Metabolism - butanoate; EC 2.6.1.19; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - propanoate; Transferase; Other Amino Acids Metabolism - beta-alanine; EC 2.6.1.22 Chromosomal Location of Human Ortholog: 16p13.2 Cellular Component: neuron projection; mitochondrion; mitochondrial matrix; 4-aminobutyrate transaminase complex Molecular Function:protein homodimerization activity; succinate-semialdehyde dehydrogenase binding; (S)-3-amino-2-methylpropionate transaminase activity; 4-aminobutyrate transaminase activity; pyridoxal phosphate binding Biological Process: response to nicotine; copulation; response to drug; synaptic transmission; neurotransmitter catabolic process; response to ethanol; behavioral response to cocaine; neurotransmitter secretion; response to hypoxia; locomotory behavior; negative regulation of blood pressure; gamma-aminobutyric acid catabolic process; response to iron ion Disease: Gaba-transaminase Deficiency
NCBI Summary:	4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P80404
NCBI GenInfo Identifier:	48429239
NCBI Gene ID:	18
NCBI Accession:	P80404.3
UniProt Secondary Accession:	P80404,Q16260, Q8N5W2, Q96BG2, Q99800, A8K386,
UniProt Related Accession:	P80404
Molecular Weight:	500
NCBI Full Name:	4-aminobutyrate aminotransferase, mitochondrial
NCBI Synonym Full Names:	4-aminobutyrate aminotransferase
NCBI Official Symbol:	ABAT
NCBI Official Synonym Symbols:	GABAT; NPD009; GABA-AT
NCBI Protein Information:	4-aminobutyrate aminotransferase, mitochondrial; GABA transferase; GABA transaminase; GABA aminotransferase; 4-aminobutyrate transaminase; gamma-amino-N-butyrate transaminase; (S)-3-amino-2-methylpropionate transaminase
UniProt Protein Name:	4-aminobutyrate aminotransferase, mitochondrial
UniProt Synonym Protein Names:	(S)-3-amino-2-methylpropionate transaminase (EC:2.6.1.22); GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; GABA-T; L-AIBAT
UniProt Gene Name:	ABAT
UniProt Entry Name:	GABT_HUMAN

	Western blot analysis of extracts of various cell lines, using ABAT Polyclonal Antibody (CAB5299) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Western blot analysis of extracts of HepG2 cells, using ABAT Polyclonal Antibody (CAB5299) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat kidney using ABAT antibody (CAB5299) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver damage using ABAT antibody (CAB5299) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using ABAT antibody (CAB5299) at dilution of 1:100 (40x lens).

Antibodies

Anti-ABAT Antibody (CAB9146)

ABAT Antibody (PACO07516)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)