Acetate Colorimetric Assay Kit (BN00882)

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SKU:
BN00882
  • Acetate Colorimetric Assay Kit (BN00882)
  • Acetate Colorimetric Assay Kit (BN00882)
€589

Description

ELISA Kit Technical ManualMSDS

Acetate Colorimetric Assay Kit

Acetate is an important component for biosynthesis. In living organisms, acetylation/deacetylation of proteins is critical for post-translational regulation of their functions. When bound to CoA, Acetyl-CoA is central to metabolism of carbohydrates and fats. It is also involved in the synthesis of biogenic compounds such as neurotransmitter acetylcholine. In Assay Genie's Acetate Assay Kit, Acetate is converted to an intermediate in the presence of Acetate Enzyme Mix & Acetate Substrate Mix. The intermediate reduces a colorless probe to a colored product with strong absorbance at 450 nm. The Acetate Colorimetric Assay Kit is rapid, simple & sensitive. It can detect less than 20 µM Acetate in various samples.

Figure 1: (a) Acetate Standard Curve. (b) Measurement of Acetate in human serum (5 μl), fish liver (~100 μg) egg (~200 μg) . Assays were performed following kit protocol.

Key Information Description

Product SKU

BN00882

Size

100 assays

Detection Method

Absorbance (450 nm)

Applications

The kit can detect less than 20 of Acetate in various samples.

Features and Benefits

  • Easy-to-use Rapid, convenient and sensitive
  • Kit contains all necessary reagents for accurate measurement of Acetate levels

Kit Components

  • Acetate Assay Buffer
  • Acetate Enzyme Mix (Lyophilized)
  • ATP (Lyophilized)
  • Acetate Substrate Mix (Lyophilized)
  • Probe (Lyophilized)
  • Acetate Standard (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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