Acetylcholinesterase Activity Colorimetric Assay Kit (BN00977)

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BN00977
  • Acetylcholinesterase Activity Colorimetric Assay Kit (BN00977)
  • Acetylcholinesterase Activity Colorimetric Assay Kit (BN00977)
€631

Description

ELISA Kit Technical ManualMSDS

Acetylcholinesterase Activity Colorimetric Assay Kit

Acetylcholinesterase (AChE) is one of the most crucial enzymes associated with nerve response and function. AChE is a serine protease that hydrolyzes the neurotransmitter acetylcholine. Irreversible inhibition of AChE may lead to muscular paralysis, convulsions, bronchial constriction, and death by asphyxiation. Assay Genie's Acetylcholinesterase Activity Assay Kit provides a simple and sensitive method for continuous monitoring of enzymatic activity using colorimetry (OD 570 nm). In this assay, AChE converts acetylcholine substrate to choline, which is then oxidized by choline oxidase (CO) to produce an intermediate. The intermediate reacts with a highly specific probe to generate color. This assay kit can detect acetylcholinesterase activity as low as 0.5 mU/ml in a variety of samples.

Figure: (a) Choline Standard Curve. (b) AChE activity in serum (1 µl) Positive Control (1 µl). Assays were performed following the kit protocol.

Key Information DEscription

Product SKU

BN00977

Size

100 assays

Detection Method

Absorbance (OD 570 nm)

Applications

  • Measurement of AChE activity in various tissues/cells
  • Screening of AChE inhibitors

Features and Benefits

  • Rapid, simple & convenient
  • This assay kit can detect acetylcholinesterase activity as low as 0.5 mU/ml in a variety of samples

Kit Components

  • AChE Assay Buffer
  • AChE Probe (DMSO)
  • AChE Substrate (Lyophilized)
  • Choline Oxidase Enzyme Mix (Lyophilized)
  • Choline Standard (Lyophilized)
  • AChE Positive Control (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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