Description
Acid Phosphatase Activity Colorimetric Assay Kit
Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease. Assay Genie's Acid Phosphatase Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (ax = 405 nm) when dephosphorylated by AP. The kit can detect µU acid phosphatase activity in samples.
Figure: (a) pNP Standard Curve (b) AP Enzyme Samples after 40 minutes following kit protocol.
Key Information | Description |
Product SKU | BN00664 |
Size | 500 assays |
Detection Method | Absorbance (405 nm) |
Species Reactivity | Mammalian |
Applications | Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined. |
Features and Benefits |
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Kit Components |
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Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |