Acid Sphingomyelinase Activity Colorimetric Assay Kit (BN00825)

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SKU:
BN00825
  • Acid Sphingomyelinase Activity Colorimetric Assay Kit (BN00825)
  • Acid Sphingomyelinase Activity Colorimetric Assay Kit (BN00825)
€707

Description

ELISA Kit Technical ManualMSDS

Acid Sphingomyelinase Activity Colorimetric Assay Kit

Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. Assay Genie's Acid Sphingomyleinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.

Figure: (a) Choline Standard Curve, (b) ASMase activity of serum and macrophage cell lysate (1 µg/µl). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00825

Size

100 assays

Detection Method

Absorbance (570 nm)

Applications

  • Measurement of ASMase activity in various tissues/cell extracts
  • Screening of ASMase inhibitors

Features and Benefits

  • Simple & Rapid Protocol
  • High-Throughput adaptable
  • This kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.

Kit Components

  • ASMase Assay Buffer I
  • ASMase Assay Buffer II
  • ASMase Substrate (Lyophilized)
  • ASMase Enzyme Mix I (Lyophilized)
  • ASMase Enzyme Mix II (Lyophilized)
  • Choline Standard (Lyophilized)
  • ASMase Positive Control (Lyophilized)
  • ASMase Probe (DMSO)

Storage Conditions

-80°C

Shipping Conditions

Dry Ice

USAGE

For Research Use Only! Not For Use in Humans.

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