Anti-ACTN1 Antibody (CAB1160)

Antibody Name:	Anti-ACTN1 Antibody
Antibody SKU:	CAB1160
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Monkey
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ACTN1 (NP_001123477.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Monkey
Positive Samples:	COS-7, PC-3, U-251MG, A673

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ACTN1 (NP_001123477.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDHY DSQQ TNDY MQPE EDWD RDLL LDPA WEKQ QRKT FTAW CNSH LRKA GTQI ENIE EDFR DGLK LMLL LEVI SGER LAKP ERGK MRVH KISN VNKA LDFI ASKG VKLV SIGA EEIV DGNV KMTL GMIW TIIL RFAI QDIS VEET SAKE GLLL WCQR KTAP YKNV NIQN FHIS WKDG LGFC ALIH RHRP ELID YGKL RKDD PLTN LNTA FDVA EKYL DIPK MLDA EDIV GTAR PDEK AIMT YVSS FYHA FSGA QKAE TAAN RICK VLAV NQEN EQLM EDYE KLAS DLLE WIRR TIPW LENR VPEN TMHA MQQK LEDF RDYR RLHK PPKV QE
Gene ID:	87
Uniprot:	P12814
Cellular Location:	Cell junction, Cell membrane, Cell projection, Cytoplasm, Z line, cytoskeleton, myofibril, ruffle, sarcomere
Calculated MW:	102kDa/103kDa/105kDa/107kDa
Observed MW:	105kDa

Synonyms:

ACTN1, BDPLT15

Background:

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ACTN1: a cytoskeletal protein of the spectrin superfamily. An actin-binding protein with multiple roles in different cell types. A nonmuscle isoform structurally similar to the erythroid beta spectrin gene.
UniProt Protein Details:	Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 14q24\|14q22-q24 Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; extracellular region; dendritic spine; fascia adherens; pseudopodium; cytosol; Z disc; ruffle; cell projection; cytoplasm; plasma membrane; stress fiber; nucleus Molecular Function:actin filament binding; integrin binding; protein domain specific binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; double-stranded RNA binding; calcium ion binding; vinculin binding Biological Process: regulation of apoptosis; actin crosslink formation; focal adhesion formation; platelet activation; actin filament bundle formation; extracellular matrix organization and biogenesis; platelet degranulation; negative regulation of cell motility; blood coagulation Disease: Bleeding Disorder, Platelet-type, 15
NCBI Summary:	Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P12814
NCBI GenInfo Identifier:	46397817
NCBI Gene ID:	87
NCBI Accession:	P12814.2
UniProt Secondary Accession:	P12814,Q1HE25, Q9BTN1, B3V8S3, B4DHH3, B7TY16,
UniProt Related Accession:	P12814
Molecular Weight:	892
NCBI Full Name:	Alpha-actinin-1
NCBI Synonym Full Names:	actinin, alpha 1
NCBI Official Symbol:	ACTN1
NCBI Official Synonym Symbols:	BDPLT15
NCBI Protein Information:	alpha-actinin-1; actinin 1 smooth muscle; non-muscle alpha-actinin-1; F-actin cross-linking protein; alpha-actinin cytoskeletal isoform
UniProt Protein Name:	Alpha-actinin-1
UniProt Synonym Protein Names:	Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1
Protein Family:	Alpha-actinin
UniProt Gene Name:	ACTN1
UniProt Entry Name:	ACTN1_HUMAN

	Western blot analysis of extracts of various cell lines, using ACTN1 antibody (CAB1160) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human liver cancer using ACTN1 antibody (CAB1160) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using ACTN1 antibody (CAB1160) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using ACTN1 antibody (CAB1160) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
ACTN1 Antibody (PACO05608)	Human Alpha-actinin-1 (ACTN1) ELISA Kit
ACTN1 Antibody (PACO07049)
ACTN1 Antibody (PACO07584)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)