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Anti-ACTR3 Antibody (CAB1064)

SKU:
CAB1064
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Monkey
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 2 Anti-ACTR3 Antibody CAB1064
  • Cell Biology Antibodies 2 Anti-ACTR3 Antibody CAB1064
€139 - €419
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Description

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Antibody Name:Anti-ACTR3 Antibody
Antibody SKU:CAB1064
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat, Monkey
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-418 of human ACTR3 (NP_005712.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:Human, Mouse, Rat, Monkey
Positive Samples:K-562, HL-60, HeLa, Jurkat, COS-1, COS-7, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-418 of human ACTR3 (NP_005712.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAGR LPAC VVDC GTGY TKLG YAGN TEPQ FIIP SCIA IKES AKVG DQAQ RRVM KGVD DLDF FIGD EAIE KPTY ATKW PIRH GIVE DWDL MERF MEQV IFKY LRAE PEDH YFLL TEPP LNTP ENRE YTAE IMFE SFNV PGLY IAVQ AVLA LAAS WTSR QVGE RTLT GTVI DSGD GVTH VIPV AEGY VIGS CIKH IPIA GRDI TYFI QQLL RDRE VGIP PEQS LETA KAVK ERYS YVCP DLVK EFNK YDTD GSKW IKQY TGIN AISK KEFS IDVG YERF LGPE IFFH PEFA NPDF TQPI SEVV DEVI QNCP IDVR RPLY KNIV LSGG STMF RDFG RRLQ RDLK RTVD ARLK LSEE LSGG RLKP KPID VQVI THHM QRYA VWFG GSML ASTP EFYQ VCHT KKDY EEIG PSIC RHNP VFGV MS
Gene ID:10096
Uniprot:P61158
Cellular Location:Cell projection, Cytoplasm, cytoskeleton
Calculated MW:47kDa
Observed MW:47kDa
Synonyms:ACTR3, ARP3
Background:The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene.
UniProt Protein Function:Arp3: Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis. Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Interacts with WHDC1. Belongs to the actin family. ARP3 subfamily.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 2q14.1

Cellular Component: Golgi membrane; Arp2/3 protein complex; focal adhesion; hemidesmosome; membrane; lamellipodium; excitatory synapse; podosome; actin filament; intercellular junction; cytosol; actin cytoskeleton

Molecular Function:actin filament binding; protein binding; structural constituent of cytoskeleton; ATP binding

Biological Process: response to antibiotic; positive regulation of filopodium formation; axon guidance; meiotic chromosome movement towards spindle pole; asymmetric cell division; regulation of myosin II filament assembly or disassembly; establishment and/or maintenance of cell polarity; ephrin receptor signaling pathway; innate immune response; cytokinesis after meiosis; positive regulation of dendrite morphogenesis; cell motility; response to carbohydrate stimulus; spindle localization

NCBI Summary:The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]
UniProt Code:P61158
NCBI GenInfo Identifier:47117647
NCBI Gene ID:10096
NCBI Accession:P61158.3
UniProt Secondary Accession:P61158,P32391, Q53QM2,
UniProt Related Accession:P61158
Molecular Weight:47,371 Da
NCBI Full Name:Actin-related protein 3
NCBI Synonym Full Names:ARP3 actin-related protein 3 homolog (yeast)
NCBI Official Symbol:ACTR3  
NCBI Official Synonym Symbols:ARP3  
NCBI Protein Information:actin-related protein 3; actin-like protein 3
UniProt Protein Name:Actin-related protein 3
UniProt Synonym Protein Names:Actin-like protein 3
UniProt Gene Name:ACTR3  
UniProt Entry Name:ARP3_HUMAN
Anti-ACTR3 Antibody (CAB1064)Western blot analysis of extracts of various cell lines, using ACTR3 antibody (CAB1064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
Anti-ACTR3 Antibody (CAB1064)Immunofluorescence analysis of HeLa cells using ACTR3 antibody (CAB1064). Blue: DAPI for nuclear staining.

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