Adenosine Deaminase Activity Assay Kit (Fluorometric) (BN00595)

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BN00595
  • Adenosine Deaminase Activity Assay Kit (Fluorometric) (BN00595)
  • Adenosine Deaminase Activity Assay Kit (Fluorometric) (BN00595)
  • Adenosine Deaminase Activity Assay Kit (Fluorometric) (BN00595)
€529

Description

ELISA Kit Technical ManualMSDS

Adenosine Deaminase Activity Assay Kit (Fluorometric)

Adenosine Deaminase (ADA) (E.C. 3.5.4.4.) is an enzyme that catalyzes the conversion of adenosine and 2'-deoxyadenosine to inosine and 2'-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and found associated with dipeptidyl peptidase-4 on the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation. In Assay Genie's ADA Activity Assay, inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA Probe to generate a fluorescent product. The kit measures total Activity of Adenosine Deaminase with limit of quantification of 10 µU recombinant Adenosine Deaminase. The range of the assay is from 10-100 µU.

Figure: (a) Inosine Standard Curve, (b) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (c) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00595

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Application

Detection of Adenosine Deaminase activity

Features and Benefits

  • Rapid, and convenient
  • The kit measures total Activity of Adenosine Deaminase with limit of quantification 10 µU recombinant Adenosine Deaminase.
  • Range of the assay: 10-100 µU.

Kit Components

  • ADA Assay Buffer (10x)
  • ADA Convertor
  • ADA Developer
  • ADA Substrate
  • ADA Probe
  • ADA Positive Control
  • Inosine Standard (10 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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