Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit (BN01023)

(No reviews yet) Write a Review
SKU:
BN01023
  • Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit (BN01023)
  • Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit (BN01023)
  • Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit (BN01023)
€931

Description

ELISA Kit Technical ManualMSDS

Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit

Adenosylhomocysteinase (AHCY) (EC 3.3.1.1) or S-adenosylhomocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. AHCY regulates the intracellular SAH concentration which in turn regulates S-adenosyl methionine (SAM)-dependent methyltransferases. Down-regulation of AHCY has been associated with certain forms of cancer and Huntington's disease, while in Wilson's disease; the enzyme is inhibited by the accumulated copper. Mutations in the AHCY gene cause SAHH deficiency disease. Assay Genie's AHCY Activity Assay kit can kinetically measure AHCY activity by detecting adenosine generation resulting from the hydrolysis of SAH. Adenosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the GenieRed Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification (L.O.Q) is 1 µU recombinant human AHCY.

Figure: (a) Adenosine Standard Curve, (b) AHCY activity in rat kidney lysate (24 μg), HeLa cell lysate (7.6 μg) and Positive Control; S: Sample, B: Background (c) AHCY specific activity in HeLa cell lysate (4 μg) and rat kidney lysate (28 μg). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN01023

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Applications

Detection of AHCY activity

Features and Benefits

  • Rapid, simple & convenient
  • The assay can detect AHCY activity as low as 1 µU recombinant human AHCY.

Kit Components

  • AHCY Assay Buffer
  • Homogenization Buffer
  • GenieRed Probe
  • SAH Substrate
  • Enzyme Mix I
  • Enzyme Mix II
  • Adenosine Standard (10 mM)
  • AHCY Enzyme (Positive Control)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose