GenieGlow ADP/ATP Ratio Assay Kit (BN00522)

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SKU:
BN00522
$849

Description

ELISA Kit Technical ManualMSDS

GenieGlow ADP/ATP Ratio Assay Kit

The changes in ADP/ATP ratio have been used to differentiate the different modes of cell death and viability. Increased levels of ATP and decreased levels of ADP have been recognized in proliferating cells. In contrast, decreased levels of ATP and increased levels of ADP are recognized in apoptotic cells. The decrease in ATP and increase in ADP are much more pronounced in necrosis than apoptosis. The ADP/ATP Ratio Assay kit utilizes bioluminescent detection of the ADP and ATP levels for a rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. ADP level is measured by its conversion to ATP that is subsequently detected using the same reaction. The assay can be fully automatic for high throughput and is highly sensitive (detects 100 mammalian cells/well).

Key Information Description

Product SKU

BN00522

Size

200 assays

Detection Methods

Luminometer or Beta Counter.

Species Reactivity

Mammalian

Applications

Bioluminescent detection of the ATP level via luciferase catalyzed reaction for a rapid screening of apoptosis and cell viability in mammalian cells.

Features and Benefits

  • Simple one-step procedure; takes only 30 minutes
  • Fast and convenient
  • The ADP/ATP ratio assay offers highly consistent results and with excellent correlation to other apoptosis markers (e.g. TUNEL-based assays and caspase assays). In addition the assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 cells/well).

Kit Compnents

  • Nucleotide Releasing Buffer
  • ATP Monitoring Enzyme
  • ADP Converting Enzyme
  • Enzyme Reconstitution Buffer

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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