ADP Colorimetric/Fluorometric Assay Kit (BN00618)

(No reviews yet) Write a Review
SKU:
BN00618
  • ADP Colorimetric/Fluorometric Assay Kit (BN00618)
  • ADP Colorimetric/Fluorometric Assay Kit (BN00618)
£625.80

Description

ELISA Kit Technical ManualMSDS

ADP Colorimetric/Fluorometric Assay Kit

ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. De-phosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. Assay Genie's newly designed ADP Assay Kit provides a convenient colorimetric and fluorometric means to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (ax = 570 nm) or fluorometric method (Ex/Em 535/587 nm). The assay is simple, sensitive, stable and high-throughput adaptable. The assay can detect as low as 1 µM ADP in biological samples.

Figure: Colorimetric and Fluorometric Standard Curves

Key Information Description

Product SKU

BN00618

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

  • In the assay, ADP is converted to ATP and pyruvate.
  • The assay can detect as low as 1 µM ADP in biological samples.

Features and Benefits

  • Simple procedure; takes less than 1 hour
  • Fast and convenient
  • The assay is sensitive, stable and high-throughput adaptable.

Kit Components

  • ADP Assay Buffer
  • ADP Probe (lyophilized)
  • DMSO (anhydrous)
  • ADP Converter
  • ADP Developer Mix
  • ADP Standard (1 µmole lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

View AllClose