Akt Activity Assay Kit (BN00679)

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SKU:
BN00679
€774

Description

ELISA Kit Technical ManualMSDS

Akt Activity Assay Kit

Akt is a protein kinase that can be activated by insulin and various growth factors and functions in a pathway involving PI3 kinase. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis. The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3as substrate. Phosphorylation of the GSK-3can be analyzed by Western blot analysis using the phospho-GSK-3specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.

Key Information Description

Product SKU

BN00679

Size

40 assays

Detection Method

Western blot analysis using anti-Phospho-GSK-3(Ser 21) antibody at 1:1000 dilutions

Species Reactivity

Mammalian

Applications

The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3as substrate.

Features and Benefits

  • Simple procedure
  • The kit is suitable for both research and drug discovery
  • The assay utilizes a N-terminal c-Jun fusion protein bead to selectively "pull down" JNK from cell lysate c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody.

Kit Components

  • Kinase Extraction Buffer
  • Akt Specific Antibody
  • Protein A Sepharose
  • GSK-3Protein/ATP Mixture
  • Kinase Assay Buffer
  • Phospho-GSK-3Specific Antibody

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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