Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit (384-Well) (BN01105)

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BN01105
  • Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit (384-Well) (BN01105)
  • Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit (384-Well) (BN01105)
  • Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit (384-Well) (BN01105)
€649

Description

ELISA Kit Technical ManualMSDS

Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit (384-Well)

Alanine transaminase (ALT) is a transaminase enzyme (EC 2.6.1.2). It is also called serum glutamic pyruvic transaminase (SGPT) or alanine aminotransferase (ALAT). ALT is found in serum and in various bodily tissues, but is usually associated with the liver. It catalyzes the reaction: ketoglutarate + alanine glutamate + pyruvate It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In Assay Genie's ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (ax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well.

Figure: (a) Pyruvate Standard Curve (colorimetric) (b) Pyruvate Standard Curve (fluorometric) (c) ALT Activity (mU/ml) in 3T3 Cell Lysate (2.5 μl, 12 mg/ml protein). Pooled Human Serum (2.5 μl), and Jurkat Cell Lysate (1.25 μl, 4 mg/ml protein). Assays were performed according to the kit protocol.

Key Information Description

Product SKU

BN01105

Size

400 assays

Kit Summary

  • Detection method - Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
  • Species reactivity - Mammalian
  • Applications - The kit detects ALT with a detection limit of 0.1 µU per well.

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Eukaryotes

Applications

Evaluation of pentose phosphate pathway

Features and Benefits

  • Simple procedure
  • Fast and convenient
  • Sensitive assays for measuring ALT activity in various biological samples and the kit is also suitable for high throughput analyses.

Kit Components

  • ALT Assay Buffer
  • GenieRed Probe
  • ALT Enzyme Mix
  • ALT Substrate
  • Pyruvate Standard
  • ALT positive control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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