Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit (BN00965)

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BN00965
  • Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit (BN00965)
  • Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit (BN00965)
  • Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit (BN00965)
  • Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit (BN00965)
€613

Description

ELISA Kit Technical ManualMSDS

Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit

Alanine transaminase (ALT) is a transaminase enzyme (EC 2.6.1.2). It is also called serum glutamic pyruvic transaminase (SGPT) or alanine aminotransferase (ALAT). ALT is found in serum and in various bodily tissues, but is usually associated with the liver. It catalyzes the reaction: ketoglutarate + alanine glutamate + pyruvate It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In Assay Genie's ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (ax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well.

Figure: a) Pyruvate Standard Curve Colorimetric b) Pyruvate Standard Curve Fluorometric c) Measurement of alanine aminotransferase activity in Positive Control d) Alanine aminotransferase activity in HepG2 Cells (10 ug) and Liver Lysate(15 ug). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00965

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Application

Evaluation of pentose phosphate pathway

Features and Benefits

  • Simple procedure
  • Fast and convenient
  • Sensitive assays for measuring ALT activity in various biological samples and the kit is also suitable for high throughput analyses.

Kit Components

  • ALT Assay Buffer
  • GenieRed Probe (in DMSO)
  • ALT Enzyme Mix
  • ALT Substrate
  • Pyruvate Standard (100 nmol/µl)
  • ALT positive control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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