Albumin (Albuminuria) Fluorometric Assay Kit (BN00780)

(No reviews yet) Write a Review
SKU:
BN00780
  • Albumin (Albuminuria) Fluorometric Assay Kit (BN00780)
  • Albumin (Albuminuria) Fluorometric Assay Kit (BN00780)
  • Albumin (Albuminuria) Fluorometric Assay Kit (BN00780)
€601

Description

ELISA Kit Technical ManualMSDS

Albumin (Albuminuria) Fluorometric Assay Kit

Albuminuria is a pathological condition characterized by the presence of high concentrations of albumin in urine. Albumin concentration ranges between 30-300 mg/L in the patient's urine sample, which is not detected by commercially available protein tests. Albuminuria has become a useful marker for the early detection of chronic kidney diseases in patients suffering from diabetes, hypertension, and cardiovascular diseases. Assay Genie's Albumin (Albuminuria) Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects albumin concentrations in healthy and Albuminuric urine samples. This assay is based on the fluorometric detection of albumin using a probe (AB580) that specifically recognizes albumin (Ex/Em: 600/630 nm). AB580 does not cause fluorescence in presence of other proteins or metabolites such as IgG, urea, ascorbic acid and glucose. Our assay can detect albumin concentrations as low as 20 mg/L.

Figure: (a) BSA Standard Curve. (b) Relative fluorescence of selected metabolites and proteins found in human urine. 1) Human Serum Albumin (30 µg) was
assayed and compared with 2) Ascorbic Acid (20 mM), 3) Glucose (20 mM), 4) Creatinine (20 mM), and 5) Urea (20 mM), 6) Lysozyme (30 µg), 7) Trypsin (30 µg), and 8) IgG (30 µg). (c) Measurement of Albumin in urine from healthy and diabetic donors (40 µl each), and saliva (30 µl). Samples were assayed following kit protocol. Albumin concentrations (in mg/L) are: healthy donor urine: 19.4; diabetic donor urine: 49.0; Saliva: 290.

Key Information Description

Product SKU

BN00780

Size

100 assays

Detection Method

Fluorescence (Ex/Em 600/630 nm)

Species Reactivity

Mammalian

Applications

Measurement of Albumin concentration in biological fluids like urine, saliva etc.

Features and Benefits

  • Simple procedure,
  • High-throughput suitable
  • Uses a specific probe (AB580) that specifically recognizes albumin
  • The assay can detect as low as 20 mg/L of Albumin in samples.

Kit Components

  • Albumin Assay Buffer
  • Albumin Diluent
  • Albumin Probe (AB580)
  • BSA Standard (2 mg/ml)

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose