Albumin-to-Creatinine Ratio (ACR) (BN00781)

(No reviews yet) Write a Review
SKU:
BN00781
  • Albumin-to-Creatinine Ratio (ACR) (BN00781)
  • Albumin-to-Creatinine Ratio (ACR) (BN00781)
  • Albumin-to-Creatinine Ratio (ACR) (BN00781)
€840

Description

ELISA Kit Technical ManualMSDS

Albumin-to-Creatinine Ratio (ACR)

Albumin-to-Creatinine Ratio (ACR) is one of the two markers used to determine chronic kidney disease (CKD). ACR is recommended to be measured on regular basis on people living with Type I and Type II diabetes. ACR is defined as the ratio between albumin (reported in mg/dl) and creatinine (reported in g/dl). This ratio estimates the amount of albumin excreted in urine during a 24 hr period. Albuminuria is diagnosed when UACR is greater than 30 mg albumin/g creatinine. Assay Genie's ACR Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects albumin (detection range: 0.02- 2.5 mg/ml), creatinine (detection range: 0.002 -0.5 mg/ml) and urine albumin-to-creatinine ratio. The ACR ratio is determined in two steps: First, albumin is determined by using a probe (AB580) that specifically recognizes albumin (Ex/Em = 600/630 nm). Second, creatinine is converted to sarcosine via enzymatic reactions. Sarcosine is specifically oxidized generating a product that reacts with a probe producing a chromophore that can be detected at 570 nm.

Figure: (a) BSA Standard Curve. (b) Creatinine Standard Curve. (c) Estimation of ACR in human urine in diabetic (1) and non-diabetic donors (2, and 3). For Albumin, 50 µl of undiluted samples and for Creatinine, 30 µl of diluted samples (100 times diluted using Creatinine Assay Buffer) were assayed following the kit protocol.

Key Information Description

Product SKU

BN00781

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 600/630 nm) & Absorbance (OD 570 nm)

Applications

  • Estimation of albumin in biological samples
  • Estimation of creatinine in biological samples
  • Determination of UACR in mammalian urine samples

Features and Benefits

  • Simple & Sensitive
  • High-Throughput adaptable
  • Suitable for detecting albumin (detection range: 0.02- 2.5 mg/ml), creatinine (detection range: 0.002 -0.5 mg/ml) and albumin-to-creatinine ratio (ACR)

Kit Components

  • Creatinine Assay Buffer
  • Albumin Assay Buffer
  • Albumin Diluent
  • Albumin Probe (AB580)
  • Creatinine Probe
  • Creatinase
  • Creatininase
  • Creatinine Enzyme Mix
  • BSA Standard (2 mg/ml)
  • Creatinine Standard (10 ol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose