Alcohol Dehydrogenase Activity Colorimetric Assay Kit (BN01000)

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SKU:
BN01000
  • Alcohol Dehydrogenase Activity Colorimetric Assay Kit (BN01000)
  • Alcohol Dehydrogenase Activity Colorimetric Assay Kit (BN01000)
€631

Description

ELISA Kit Technical ManualMSDS

Alcohol Dehydrogenase Activity Colorimetric Assay Kit

Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation. Assay Genie's Alcohol Dehydrogenase Assay Kit provides a convenient tool for sensitive detection of the ADH in a variety of samples. In the assay ADH utilizes ethanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (= 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.

            (a)

(b)

Figure: (a) NADH Standard curve obtained using kit protocol. (b) Sample test curve * Sample: Bovine liver extraction (36µg protein)

Key Information Description

Product SKU

BN01000

Size

100 assays

Detection Method

Absorbance (450 nm)

Species Reactivity

Mammalian

Applications

This assay detects ADH activity as low as 0.01 mU in samples.

Features and Benefits

  • Simple procedure
  • Fast and convenient

Kit Components

  • ADH Assay Buffer
  • Substrate
  • Developer (Lyophilized)
  • ADH Positive Control (Lyophilized)
  • NADH Standard (0.5 ol, Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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