Alkaline Phosphatase Activity Assay Kit (BN00665)

SKU:
BN00665
Product type:
Cellular Assay
Detection Method:
Colorimetric
  • Cell Biology Assays Alkaline Phosphatase Activity Assay Kit BN00665
  • Cell Biology Assays Alkaline Phosphatase Activity Assay Kit BN00665
  • Cell Biology Assays Alkaline Phosphatase Activity Assay Kit BN00665
€619

Description

Technical Manual MSDS

Alkaline Phosphatase Activity Assay Kit

The Assay Genie Alkaline Phosphatase Activity Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay designed to measure Alkaline Phosphatase (ALP) activity in serum and biological samples. It contains 10 substrate tablets providing convenience for multiple usages. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (ax= 405 nm) when dephosphorylated by ALP. The Kit can detect 10-250 µU ALP in samples.

Alkaline Phosphatase Activity Assay Kit Data

Assay Information Description
Product Name: Alkaline Phosphatase Activity Assay Kit Data
Product SKU: BN00665
Size: 500 assays
Detection Method: Colorimetric (Absorbance 405 nm)
Sensitivity: 0-250 µU
Assay Time: ˜3 hours
Storage: Store the kit at -20°C, protect from light
Shipping Conditions: Gel Pack
Usage: For research use only. Not for use in humans.

Alkaline Phosphatase Activity Assay Kit Components

Component Quantity
ALP Assay Buffer 100 ml
pNPP (10 TAB) 1 vial
ALP Enzyme 1 vial
Stop Solution 10 ml

Product Images

Figure: a. pNP Standard Curve. b. Measurement of ALP activity in fresh medium (80 μl, without culturing), 3-day old HeLa cell culture medium (80 μl), and human serum (80 μl, 1:10 diluted). c. Measurement of ALP activity in HeLa cells: 5X10 4 HeLa cells were homogenized in 1 ml of Assay Buffer, diluted 1:10 in Assay Buffer and 80 μl was used to measure ALP activity. Assays were performed following the kit protocol.

Alkaline Phosphatase Background

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone.

View AllClose