Alkaline Phosphatase Activity Colorimetric Assay Kit (BN00665)

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SKU:
BN00665
  • Alkaline Phosphatase Activity Colorimetric Assay Kit (BN00665)
  • Alkaline Phosphatase Activity Colorimetric Assay Kit (BN00665)
  • Alkaline Phosphatase Activity Colorimetric Assay Kit (BN00665)
€619

Description

ELISA Kit Technical ManualMSDS

Alkaline Phosphatase Activity Colorimetric Assay Kit

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone. Assay Genie's Alkaline Phosphatase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay designed to measure ALP activity in serum and biological samples. It contains 10 substrate tablets providing convenience for multiple usages. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (ax= 405 nm) when dephosphorylated by ALP. The Kit can detect 10-250 µU ALP in samples.

Figure: a. pNP Standard Curve. b. Measurement of ALP activity in fresh medium (80 μl, without culturing), 3-day old HeLa cell culture medium (80 μl), and human serum (80 μl, 1:10 diluted). c. Measurement of ALP activity in HeLa cells: 5X10 4 HeLa cells were homogenized in 1 ml of Assay Buffer, diluted 1:10 in Assay Buffer and 80 μl was used to measure ALP activity. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00665

Size

500 assays

Detection Method

Absorbance (405 nm)

Species Reactivity

Mammalian

Applications

The Kit can detect 10-250 µU ALP in samples.

Features and Benefits

  • Simple procedure; takes ~ 3 hours
  • Fast and convenient
  • The assay is sensitive and stable
  • The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.

Kit Components

  • ALP Assay Buffer
  • pNPP (10 TAB)
  • ALP Enzyme
  • Stop Solution

Storage Conditions  

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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