Alpha Galactosidase (alpha-Gal) Activity Assay Kit (Fluorometric) (BN00660)

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BN00660
  • Alpha Galactosidase (alpha-Gal) Activity Assay Kit (Fluorometric) (BN00660)
  • Alpha Galactosidase (alpha-Gal) Activity Assay Kit (Fluorometric) (BN00660)
  • Alpha Galactosidase (alpha-Gal) Activity Assay Kit (Fluorometric) (BN00660)
€607

Description

ELISA Kit Technical ManualMSDS

Alpha Galactosidase (alpha-Gal) Activity Assay Kit (Fluorometric)

Alpha-Galactosidase (Gal; EC 3.2.1.22) hydrolyzes alpha-galactosyl moieties found in glycolipids and glycoproteins. In mammals, Gal hydrolyzes poly- and oligosaccharides commonly found in dietary sources that are difficult to digest. Therefore, Gal is used in dietary supplements that help to reduce the production of intestinal gases due to consumption of certain foods. It is known total Gal activity is due to two major isozymes with unique, yet different thermostability profiles. Alpha-Galactosidase A, is thermolabile and represents approximately 90% of total Gal activity. Fabry Disease, a lysosomal disease disorder, is characterized by mutations in alpha-Galactosidase A. These mutations cause abnormal accumulation of glycosphingolipids in lysosomes. Assay Genie's Alpha Galactosidase Activity Assay Kit provides a simple, rapid way to monitor total Gal activity in wide variety of biological samples. In this kit, Gal cleaves a synthetic specific substrate releasing a fluorophore, which can be easily quantified (Ex/Em= 360/445 nm). The assay is specific, sensitive and can detect as low as 0.1 µU of Galactosidase activity.

Figure: (a) 4-Methylumbelliferon Standard Curve. Results are from multiple experiments. (b) α-Galactosidase Activity in Mouse Kidney Tissue Extracts (1 µg protein) and U937 Cell Lysates (0.2 µg protein). (c) Measurement of α-Galactosidase Activity in undiluted Human Pooled Saliva (5 µl). All assays were performed following kit protocol.

Key Information Description

Product SKU

BN00660

Size

100 assays

Kit Summary

  • Detection method: Fluorescence (Ex/Em = 360/445 nm)
  • Application:Measurement of Galactosidase activity in various samples

Detection Method

Fluorescence (Ex/Em = 360/445 nm)

Species Reactivity

Eukaryotes

Applications

Measurement of Galactosidase activity in various samples

Features and Benefits

  • Quick and easy method to measure Alpha Galactosidase activity.
  • Detection limit: < 0.1 µU.
  • Specific: does not detect beta galactosidase activity

Kit Components

  • Gal Assay Buffer
  • Gal Stop Solution
  • Gal Substrate
  • 4-Methylumbelliferone Standard Gal
  • Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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