Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit (BN00901)

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BN00901
  • Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit (BN00901)
  • Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit (BN00901)
€619

Description

ELISA Kit Technical ManualMSDS

Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit

Ketoglutarate (KG) is a key intermediate in the Krebs cycle, coming after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing KG from transamination of glutamate, or through the action of glutamate dehydrogenase. KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of inborn errors of metabolism. Assay Genie's Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying KG in a variety of samples. In the assay, ketoglutarate is transaminated with the generation of pyruvate which is utilized to convert a nearly colorless probe to both color (ax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The Ketoglutarate Assay Kit is useful for detecting ketoglutarate in the range of 0.01 to 10 nmoles.

Figure 1: α-Ketoglutarate standard curve generated using this kit protocol.

Key Information Description

Product SKU

BN00901

Size

100 assays

Detection Methods

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

The Ketoglutarate Assay Kit is useful for detecting ketoglutarate in the range of 0.01 to 10 nmoles.

Features and Benefits

  • Simple procedure; takes ~ 30 minutes to complete the assay
  • Fast and convenient
  • Highly sensitive assays for measuring alpha-Ketaglutarate levels in biological samples

Kit Components

  • KG Assay Buffer
  • KG GenieRed Probe (in DMSO)
  • KG Converting Enzyme
  • KG Development Enzyme Mix
  • KG Standard (10 µmol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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