Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit (BV0002)

alpha-Ketoglutarate (alpha-KG) is a key intermediate in the Krebs cycle, coming after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing alpha-KG from transamination of glutamate, or through the action of glutamate dehydrogenase. alpha-KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of inborn errors of metabolism. Assay Genie's alpha-Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying alpha-KG in a variety of samples. In the assay, alpha-ketoglutarate is transaminated with the generation of pyruvate which is utilized to convert a nearly colorless probe to both color (Abs»max = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The alpha-Ketoglutarate Assay Kit is useful for detecting alpha-ketoglutarate in the range of 0.01 to 10 nmoles.