Amylase Activity Colorimetric Assay Kit (BN00931)

SKU:
BN00931
  • Metabolism Assays Amylase Activity Colorimetric Assay Kit BN00931
  • Metabolism Assays Amylase Activity Colorimetric Assay Kit BN00931
€476

Description

ELISA Kit Technical ManualMSDS

Amylase Activity Colorimetric Assay Kit

Amylases are enzymes that break starch down to sugar molecules. amylase is the major form of amylase found in humans and other mammals as well as an enzyme present in seeds, or in fungi (baker's yeast for instance). amylase is a calcium metalloenzyme, completely unable to function in the absence of calcium. In human physiology, both the salivary and pancreatic amylases are major digestive enzymes. Increased enzyme levels in humans are associated with salivary trauma; mumps due to inflammation of the salivary glands, pancreatitis and renal failure. A simple, direct and automation-ready procedure for measuring amylase activity is, therefore, very desirable. Assay Genie's amylase Assay uses ethylidene-pNP-G7 as the substrate. Once the substrate has been specifically cleaved by amylase, the smaller fragments produced can be acted upon by glucosidase, which causes the ultimate release of the chromophore that can then be measured at 405 nm. The assay can detect amylase content as low as 0.2 mU.

Figure: Sample test and Nitrophenol Standard Curve

Key Information Description

Product SKU

BN00931

Size

100 assays

Detection Method

Absorbance (405 nm)

Species Reactivity

Mammalian

Applications

The assay can detect amylase content as low as 0.2 mU.

Features and Benefits

  • Simple procedure; takes ~ less than 40 minutes
  • Fast and convenient

Kit Components

  • Amylase Assay Buffer
  • Amylase Substrate Mix
  • Amylase Positive Control
  • Nitrophenol standard (2 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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