Anti-Aquaporin 4 Antibody (CAB13926)

Antibody Name:	Anti-Aquaporin 4 Antibody
Antibody SKU:	CAB13926
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 244-323 of human Aquaporin 4 (NP_001641.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, SKOV3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 244-323 of human Aquaporin 4 (NP_001641.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AGGL YEYV FCPD VEFK RRFK EAFS KAAQ QTKG SYME VEDN RSQV ETDD LILK PGVV HVID VDRG EEKK GKDQ SGEV LSSV
Gene ID:	361
Uniprot:	P55087
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	32kDa/34kDa
Observed MW:	35kDa

Synonyms:

AQP4, MIWC, WCH4, aquaporin-4

Background:

This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.

UniProt Protein Function:	AQP4: a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The predominant aquaporin found in brain. Two alternatively spliced transcript variants have been described
UniProt Protein Details:	Protein type:Transporter, aquaporin family; Membrane protein, integral; Channel, misc.; Membrane protein, multi-pass; Transporter Chromosomal Location of Human Ortholog: 18q11.2-q12.1 Cellular Component: basolateral plasma membrane; cytoplasm; external side of plasma membrane; integral to membrane; plasma membrane Molecular Function:glycerol channel activity; water channel activity; water transporter activity Biological Process: glycerol transport; multicellular organismal water homeostasis; renal water homeostasis; transmembrane transport; transport; water transport
NCBI Summary:	This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015]
UniProt Code:	P55087
NCBI GenInfo Identifier:	2506859
NCBI Gene ID:	361
NCBI Accession:	P55087.2
UniProt Secondary Accession:	P55087,P78564,
UniProt Related Accession:	P55087
Molecular Weight:	32,300 Da
NCBI Full Name:	Aquaporin-4
NCBI Synonym Full Names:	aquaporin 4
NCBI Official Symbol:	AQP4
NCBI Official Synonym Symbols:	MIWC; WCH4
NCBI Protein Information:	aquaporin-4
UniProt Protein Name:	Aquaporin-4
UniProt Synonym Protein Names:	Mercurial-insensitive water channel; MIWC; WCH4
Protein Family:	Aquaporin
UniProt Gene Name:	AQP4
UniProt Entry Name:	AQP4_HUMAN

	Western blot analysis of extracts of various cell lines, using Aquaporin 4 antibody (CAB13926) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using Aquaporin 4 antibody (CAB13926) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using Aquaporin 4 antibody (CAB13926) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using Aquaporin 4 antibody (CAB13926) at dilution of 1:50 (40x lens).

Antibodies	ELISA Kits
Anti-Aquaporin 4 Antibody (CAB2887)	Human AQP4 / Aquaporin-4 ELISA Kit
Anti-AQP4 Antibody (CAB11210)	Human AQP-4 (Aquaporin 4) CLIA Kit (HUES00352)
Anti-Aquaporin 4 Antibody (CAB5665)	Human AQP-4 (Aquaporin 4) ELISA Kit (HUES01694)
AQP4 Antibody (PACO00414)	Human Aquaporin-4 (AQP4) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)