Anti-Tag Antibodies

GFP (Green Fluorescent Protein)

Fluorophore

Isolated from the jellyfish Aequorea victoria. Yields a green colour when exposed to blue or ultraviolet (UV) light.

Reporter assays, fluorescence microscopy, protein colocalization. macrophotography, transgenics.

RFP (Red Fluorescent Protein)

Fluorophore

The original RFP was isolated from Discosoma, and named DsRed. Yields a red-orange colour when excited.

Protein localization studies (detection), protein purification, optical imaging.

T7 (T7 RNA Polymerase)

Fluorophore

RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA from DNA in the 5'→ 3' direction.

Protein detection and expression - tag in many expression vectors (based on T7 RNA polymerase expression system).

mCherry

Fluorophore

Member of the mFruits family of monomeric red fluorescent proteins. Derived from DsRed of Discosoma sea anemones.

Fluorescence microscopy, long-wavelength hetero-FRET (fluorescence resonant energy transfer) , bacteria labeling.

DDDDK

Fluorophore

Hydrophillic polypeptide tag.

Protein localization via immunofluorescence, SDS-Page.

HA

Peptide

Tag derived from hemagglutinin 98-106 amino acids in length. Hemagglutinin is a class I fusion protein found on the surface of influenza viruses.

Protein detection and purification, epitope tag in expression vectors.

His

Peptide

Tag derived from the amino acid histidine. Vary in length between 4 and 10 residues, with the most common His-tag being 6 residues in length (Hexahistidine tag).

Detection of protein-protein interactions, protein purification via binding assays, fluorescent microscopy (fluorohistidine tags).

Myc

Peptide

Tag derived from the c-myc gene product.

Affinity Chromatography, cellulite localization studies by immunofluorescence, Protein detection by Western blotting.

GST (Glutathione-S-transferase)

Polyeptide

Large tag consisting of 220 amino acids (roughly 26 kDa).

Protein purification via pull-down assays.

TAP (Transporter associated with antigen processing)

Polyeptide

Consists of two Immunoglobulin G (IgG) binding domains from the Staphylococcus aureus surface protein, Protein A and a calmodulin-binding peptide (CBP).

Protein purification via protein A affinity chromatography.

Trx (Thioredoxin)

Polyeptide

Thioredoxin is normally involved in the reduction of oxidised cysteine residues as well as the cleavage of disulfide bonds. Used as a solubilization tag.

Assist protein folding, improve solubility of tagged protein.

V5

Polyeptide

Short epitope tag approximately 14 amino acids in length.

Protein detection and purification.

MBP (Maltose-binding protein)

Polyeptide

Protein tag expressed in E.Coli responsible for the uptake and efficient catabolism of maltodextrins. Approximately 42.5 kDa in length

Protein purification by affinity chromatography.