TUNEL Assay Kit (BrdU-IHC DNA) (BN00656)

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SKU:
BN00656
€900

Description

ELISA Kit Technical ManualMSDS

TUNEL Assay Kit (BrdU-IHC DNA)

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. Assay Genie's Apo-BrdU-IHC™ Kit is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled antiBrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, HUrea tablets for color generation and methyl green solution for counter staining the cells.

Key Information Description

Product SKU

BN00656

Size

50 assays

Detection Method

Mammalian

Applications

The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation

Features and Benefits

  • Simple procedure; takes ~ 3 hours
  • Fast and convenient
  • The assay is sensitive and stable
  • The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.

Kit Components

  • Control Slides-pos/neg
  • Blocking Buffer
  • HUrea Tablets
  • Protease K
  • DAB Tablets
  • TdT Enzymes
  • Br-dUTP
  • 200X Conjugate
  • 5X Reaction Buffer
  • Anti-BrdU-Biotin Antibody
  • Methyl Green

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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