Apoptotic DNA Ladder Isolation Kit (BN00450)

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SKU:
BN00450
€541

Description

ELISA Kit Technical ManualMSDS

Apoptotic DNA Ladder Isolation Kit

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. Assay Genie's Apoptotic DNA Ladder Extraction Kit provides an easy and sensitive means for detecting DNA fragmentation in apoptotic cells. The new procedure selectively extract DNA ladders without interference of intact genomic DNA, which significantly increase the cell numbers that can be can be extracted and load on one agarose gel well, therefore increase the detection sensitivity.

Figure: DNA ladders were extracted from 2x106 Jurkat cells treated with 2µM Camptothes, according to kit instructions. The kit extracts laddered DNA only, not the intact genomics, therefore more cells can be extracted and load on the agarose gel. Lane 1: 1 kb DNA ladder; Lane 2: 6 hour induction; Lane 3: 12 hour induction.

Key Information Description

Product SKU

BN00450

Size

50 assays

Detection Method

Agarose gel electrophoresis (with Ethidium Bromide)

Species Reactivity

Mammalian

Applications

Extraction of apoptotic DNA ladders without interference of intact genomic DNA

Features and Benefits

  • Simple one-step procedure; takes only 2-4 hours
  • Fast and convenient
  • The kit can extract apoptotic DNA ladder from both tissues and cells with as little as 5% or less apoptotic cell population.

Kit Components

  • DNA Ladder Extraction Buffer
  • Enzyme A Solution
  • Enzyme B (Lyophilized)
  • Ammonium Acetate Solution
  • DNA Suspension Buffer

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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