Ascorbic Acid Colorimetric/Fluorometric Assay Kit (BN00885)

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SKU:
BN00885
€565

Description

ELISA Kit Technical ManualMSDS

Ascorbic Acid Colorimetric/Fluorometric Assay Kit

Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. It is important to be able to monitor ascorbic acid content in these different samples. Assay Genie's Ascorbic Acid Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples. In this assay, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid can be easily determined by either colorimetric (spectrophotometry at = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect 0.01-10 nmol of ascorbic acid per assay in various samples.

Figure: Standard Curve obtained using kit protocol

Key Information Description

Product SKU

BN00885

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

The assay can detect 0.01-10 nmol of ascorbic acid per assay in various samples.

Features and Benefits

  • Simple procedure; takes ~ 5 minutes for the reaction to complete
  • Fast and convenient

Kit Components

  • Ascorbic Acid Assay Buffer
  • Ascorbic Acid Probe (in DMSO)
  • Catalyst
  • Ascorbic Acid Enzyme Mix (lyophilized)
  • Ascorbic Acid Standard (20 µmole)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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