Asparaginase Activity Colorimetric/Fluorometric Assay Kit (BN00967)

(No reviews yet) Write a Review
SKU:
BN00967
  • Asparaginase Activity Colorimetric/Fluorometric Assay Kit (BN00967)
  • Asparaginase Activity Colorimetric/Fluorometric Assay Kit (BN00967)
  • Asparaginase Activity Colorimetric/Fluorometric Assay Kit (BN00967)
£592.02

Description

ELISA Kit Technical ManualMSDS

Asparaginase Activity Colorimetric/Fluorometric Assay Kit

Asparaginase (EC 3.5.1.1) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs). The enzyme has been used to reduce acrylamide, a suspected carcinogen, produced in fried starchy food products and to treat acute lymphoblastic leukemia (ALL) and some other hematopoietic neoplasms (e.g. multiple myeloma). Metabolization of asparagine prevents acrylamide formation in fried foods (Maillard reaction). The enzyme's antineoplastic effects are based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine. However, the enzyme is not without some antigenicity and toxicity so it is very important to measure its activity in biological samples or monitor its activity during therapy. Assay Genie provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples. In the assay, Asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected colorimetrically (570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.

Figure: (a) Aspartate Standard curve (colorimetric detection, 0-10 nmole/well). One mole of aspartate corresponds to one mole of asparagine metabolized by asparaginase. (b) Aspartate Standard curve (fluorometric detection, 0-1 nmole/well). (c) Reaction kinetics of asparaginase activity in human serum spiked with various amounts of asparaginase from E. coli. Pooled normal serum (each 5 μl per well) spiked with 0, 20, 80 or 160 mU/ml of asparaginase was assayed in colorimetric mode according to the kit protocol. Calculated sample asparaginase activities were 0.28 mU/ml, 20.28 mU/ml, 76.15 mU/ml and 153.9 mU/ml, respectively. Spike recovery ranged from 95 – 102%.

Key Information Description

Product SKU

BN00967

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/590 nm)

Applications

N/A

Features and Benefits

  • Simple procedure; takes 40 minutes
  • Fast, reliable and convenient
  • Kit can be used to detect Asparaginase enzyme's antineoplastic effects, based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine.

Kit Components

  • Asparaginase Assay Buffer
  • GenieRed Probe (in DMSO)
  • Substrate Mix
  • Aspartate Enzyme Mix
  • Conversion Mix
  • Positive Control
  • Aspartate Standard (100 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose