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Anti-ATF4 Antibody (CAB0201)

SKU:
CAB0201
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 1 Anti-ATF4 Antibody CAB0201
  • Cell Biology Antibodies 1 Anti-ATF4 Antibody CAB0201
€139 - €419
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Description

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Antibody Name:Anti-ATF4 Antibody
Antibody SKU:CAB0201
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ATF4 (NP_001666.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ATF4 (NP_001666.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTEM SFLS SEVL VGDL MSPF DQSG LGAE ESLG LLDD YLEV AKHF KPHG FSSD KAKA GSSE WLAV DGLV SPSN NSKE DAFS GTDW MLEK MDLK EFDL DALL GIDD LETM PDDL LTTL DDTC DLFA PLVQ ETNK QPPQ TVNP IGHL PESL TKPD QVAP FTFL QPLP LSPG VLSS TPDH SFSL ELGS EVDI TEGD RKPD YTAY VAMI PQCI KEED TPSD NDSG ICMS PESY LGSP QHSP STRG SPNR SLPS PGVL CGSA RPKP YDPP GEKM VAAK VKGE KLDK KLKK MEQN KTAA TRYR QKKR AEQE ALTG ECKE LEKK NEAL KERA DSLA KEIQ YLKD LIEE VRKA RGKK RVP
Gene ID:468
Uniprot:P18848
Cellular Location:Cell membrane, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:38kDa
Observed MW:50kDa
Synonyms:ATF4, CREB-2, CREB2, TAXREB67, TXREB
Background:This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.
UniProt Protein Function:ATF-4: a transcription factor that is a member of the leucine zipper family. Isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). Involved in cisplatin resistance. Other members of the family include AP-1 transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; neuron projection; cytoplasm; microtubule organizing center; nucleus

Molecular Function:protein C-terminus binding; leucine zipper domain binding; protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity

Biological Process: amino acid metabolic process; transcription from RNA polymerase II promoter; positive regulation of apoptosis; unfolded protein response; positive regulation of transcription, DNA-dependent; negative regulation of potassium ion transport; positive regulation of transcription from RNA polymerase I promoter; gluconeogenesis; cellular response to glucose starvation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; gamma-aminobutyric acid signaling pathway

NCBI Summary:This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]
UniProt Code:P18848
NCBI GenInfo Identifier:116241262
NCBI Gene ID:468
NCBI Accession:P18848.3
UniProt Secondary Accession:P18848,Q9UH31,
UniProt Related Accession:P18848
Molecular Weight:Calculated Molecular Weight: 38kDaObserved Molecular Weight: 38kDa
NCBI Full Name:Cyclic AMP-dependent transcription factor ATF-4
NCBI Synonym Full Names:activating transcription factor 4
NCBI Official Symbol:ATF4  
NCBI Official Synonym Symbols:CREB2; TXREB; CREB-2; TAXREB67  
NCBI Protein Information:cyclic AMP-dependent transcription factor ATF-4; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; cyclic AMP-responsive element-binding protein 2; tax-responsive enhancer element-binding protein 67
UniProt Protein Name:Cyclic AMP-dependent transcription factor ATF-4
UniProt Synonym Protein Names:Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67
Protein Family:Cyclic AMP-dependent transcription factor
UniProt Gene Name:ATF4  
UniProt Entry Name:ATF4_HUMAN
Anti-ATF4 Antibody (CAB0201)Western blot analysis of extracts of HeLa cells, using ATF4 antibody (CAB0201) at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 ug/mL) for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
Anti-ATF4 Antibody (CAB0201)Immunofluorescence analysis of HeLa cells using ATF4 antibody (CAB0201) at dilution of 1:100. Blue: DAPI for nuclear staining.

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