ATP Colorimetric Assay Kit (384-well) (BN01119)

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SKU:
BN01119
  • ATP Colorimetric Assay Kit (384-well) (BN01119)
  • ATP Colorimetric Assay Kit (384-well) (BN01119)
€679

Description

ELISA Kit Technical ManualMSDS

ATP Colorimetric Assay Kit (384-well)

Adenosine Triphosphate (ATP) is a derivative of adenosine nucleotide that contains a large amount of biochemical energy stored in its high-energy phosphate bonds. ATP releases energy when it is hydrolyzed into ADP or AMP (Adenosine Di or Mono Phosphate). This energy is used by cells to drive various cellular events like metabolic processes, enzymatic reactions, motility, cell division and muscle contraction. It is also used as a substrate in signal transduction, by kinases to phosphorylate proteins and lipids, and by adenylate cyclase to produce cyclic AMP. In eukaryotic cells, ATP is produced via cellular respiration in mitochondria and by photosynthesis in chloroplasts. Anaerobic bacteria can produce ATP by breaking down nutrients into simpler metabolites, and then using the released energy to form ATP from ADP and Pi (inorganic phosphate). Aerobic bacteria also make ATP from ADP and Pi by ATPase which takes the advantage of 'proton motive force' when protons travel back to cytosol from periplasmic space. There are many commercially available kits that detects ATP in femtomoles or less by using luminescence but these products require specialized instrumentation and utilize luciferase which can be difficult to maintain in active form. Assay Genie's ATP Colorimetric Assay Kit is designed to be a robust, simple, stable, colorimetric method which utilizes a series of enzymatic reactions to form a product that is easily quantified at OD 570 nm. The method is rapid, simple, sensitive and designed for high-throughput format using a 384-well plate. The kit can detect as low as 80 pmol of ATP in a 384 well assay plate.

Figure. a) ATP Standard Curve b) Quantitation of ATP in previously frozen (stored at -80°C) rat leg muscle and fresh Jurkat cells. Samples were processed following kit’s protocol and 5 µl loaded in the well. Assays were performed following kit’s protocol. As expected, a low yield of ATP was observed from the frozen rat muscle tissue sample.

Key Information Description

Product SKU

BN01119

Size

400 assays

Detection Method

Absorbance (570 nm)

Species Reactivity

All

Applications

Assay Genie's ATP Assay Kit provides a convenient tool for sensitive detection of the intracellular nucleotides ATP

Features and Benefits

  • Simple procedure; takes less than 1 hour
  • Fast and convenient
  • Kit is designed to be a robust method which enzymatic detection of ATP in numerous biological samples

Kit Components

  • ATP Assay Buffer
  • ATP Probe
  • ATP Converter (lyophilized)
  • Developer Mix (lyophilized)
  • ATP Standard (1 µmol; lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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