ATP Colorimetric/Fluorometric Assay Kit (BN00617)

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SKU:
BN00617
  • ATP Colorimetric/Fluorometric Assay Kit (BN00617)
  • ATP Colorimetric/Fluorometric Assay Kit (BN00617)
  • ATP Colorimetric/Fluorometric Assay Kit (BN00617)
€667

Description

ELISA Kit Technical ManualMSDS

ATP Colorimetric/Fluorometric Assay Kit

ATP is the primary energy currency of living systems. Virtually all energy requiring processes utilize the chemical energy stored in the phosphate bond of ATP. ATP is formed exclusively in the mitochondria and a variety of genetic diseases can affect ATP formation in the mitochondria. There are a number of commercially available ATP assays which detects femtomoles or less of ATP by measuring luminescence (Assay Genie Kit 254-200, for example) but these kits require specialized luminescence instrumentation and utilize luciferase which can be difficult to maintain in active form. Assay Genie newly developed ATP Colorimetric and Fluorometric Assay kit is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (ax = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect as low as 50 picomol (1 µM) of ATP in various samples. The kit provides sufficient reagents for 100 assays.

Figure. a) ATP Standard Curves, Colorimetric. b) Fluorometric. c) Quantitation of ATP in fish liver (2.5 µl of 10 times diluted sample), fish muscle (5 µl of 10 times diluted sample) and Jurkat cell lysate (5 ul) using fluorometric assay. Samples were spiked with known amounts of ATP (300 pmol). Assays were performed according to the kit protocol.

Key Information Description

Product SKU

BN00617

Size

100 assays

Detection Methods

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

All

Applications

The assay can detect as low as 50 picomol (1 µM) of ATP in various samples.

Features and Benefits

  • Simple procedure; takes less than 1 hour
  • Fast and convenient
  • Kit is designed to be a robust method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified.

Kit Components

  • ATP Assay Buffer
  • ATP Probe (in DMSO)
  • ATP Converter
  • Developer Mix (lyophilized)
  • ATP Standard (1 µmol; lyophilized)

Storage Conditions

-20°C

Shipping Condidtions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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