BCA Protein Assay Kit (Test Tube) - Reducing Agent Compatible (BN01035)

SKU:
BN01035
  • Molecular Biology Tools BCA Protein Assay Kit Test Tube - Reducing Agent Compatible BN01035
  • Molecular Biology Tools BCA Protein Assay Kit Test Tube - Reducing Agent Compatible BN01035
  • Molecular Biology Tools BCA Protein Assay Kit Test Tube - Reducing Agent Compatible BN01035
€434

Description

ELISA Kit Technical ManualMSDS

BCA Protein Assay Kit (Test Tube) - Reducing Agent Compatible

Assay Genie's Reducing Agent Compatible BCA Protein Assay Kit is the only protein estimation kit on the market that is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM & Beta-mercaptoethanol up to 35 mM. This kit is adapted from Assay Genie's BCA Assay Kit II (cat. # K813) and is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in alkaline conditions. Reducing agents in samples interfere with BCA assay due to their ability to reduce Cu+2. Assay Genie's Reducing Agent Compatible BCA Protein Assay Kit is useful for determining protein concentrations of samples containing DTT, TCEP & Beta-mercaptoethanol. The assay is linear over the widest range of protein concentration between 25-2000 /ml. In general, protein concentrations are calculated with reference to a commonly used protein standard. The kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.

Figure: Standard Curves for BSA containing blocking reagent in the presence and absence of (a) 10 mM DTT ,(b) 20 mM TCEP and (c)35 mM β-mercaptoethanol (BME) . The experiments were performed in test tubes as outlined in the protocol given above.

Key Information Description

Product SKU

BN01035

Size

250 assays

Detection Method

Absorbance (562 nm)

Applications

Measuring total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.

Features and Benefits

  • Simple & convenient.
  • Optimized for determining total protein content of samples containing strong reducing agents such as DTT (10 mM), TCEP (20 mM) & Beta-mercaptoethanol (35 mM).
  • The assay is linear over a wide range of protein concentration (25-2000 /ml).

Kit Components

  • BCA Reagent A
  • BCA Reagent B
  • BSA Standard (2 mg/ml)
  • Blocking Reagent (20 mg/tube)
  • Blocking Reagent Buffer

Storage Conditions

RT

Shipping Conditions

RT

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Molecular Tools
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