Anti-BES1 Antibody (CAB16262)

Antibody Name:	Anti-BES1 Antibody
Antibody SKU:	CAB16262
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Arabidopsis thaliana, Rice
Host Species:	Rabbit
Immunogen:	Recombinant protein of human BES1

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Arabidopsis thaliana, Rice
Positive Samples:

Immunogen:	Recombinant protein of human BES1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	838518
Uniprot:	Q9LN63
Cellular Location:
Calculated MW:	36kDa
Observed MW:	Refer to figures

Synonyms:

BES1, BZR2, 107 PROTEIN, BRASSINAZOLE-RESISTANT 2, BRI1-EMS-SUPPRESSOR 1, F18O14.7, F18O14_7

Background:

Encodes brassinosteroid (BR) signalling protein that accumulates in the nucleus as dephosphorylated form in response to BRs. Is phosphorylated by the BIN2 GSK3 kinase. It synergistically interacts with BIM1 to bind to E box sequences (CANNTG). The protein contains a nuclear localization signal (NLS), followed by a highly conserved amino-terminal domain (N) shared by all family members, a BIN2 phosphorylation domain (P), a PEST motif, involved in protein degradation in the absence of BR, and a carboxyl-terminal domain. BES1 can interact with the ELF6 and REF6 Jumonji N/C-domain containing proteins and may direct them to modify histone methylation upstream of some brassinosteroid responsive-genes. Works with BRAVO to regulate QC division in the root.

UniProt Protein Function:	Positive regulator of brassinosteroid (BR) signaling. Transcription factor that activates target gene expression by binding specifically to the DNA sequence 5'-CANNTG-3'(E box) through its N-terminal domain. Can bind individually to the promoter as a homodimer or synergistically as a heterodimer with BIM1, BIM2 or BIM3. The C-terminal domain is probably involved in transcriptional activation (PubMed:12007405, PubMed:15680330, PubMed:18467490, PubMed:19170933). Recruits the transcription elongation factor IWS1 to control BR-regulated gene expression (PubMed:20139304). Forms a trimeric complex with IWS1 and ASHH2/SDG8 to regulate BR-regulated gene expression (PubMed:24838002). Promotes quiescent center (QC) self-renewal by cell divisions in the primary root. Binds to the E-boxes of the BRAVO promoter to repress its expression (PubMed:24981610).
NCBI Summary:	Encodes brassinosteroid (BR) signalling protein that accumulates in the nucleus as dephosphorylated form in response to BRs. Is phosphorylated by the BIN2 GSK3 kinase. It synergistically interacts with BIM1 to bind to E box sequences (CANNTG). The protein contains a nuclear localization signal (NLS), followed by a highly conserved amino-terminal domain (N) shared by all family members, a BIN2 phosphorylation domain (P), a PEST motif, involved in protein degradation in the absence of BR, and a carboxyl-terminal domain. BES1 can interact with the ELF6 and REF6 Jumonji N/C-domain containing proteins and may direct them to modify histone methylation upstream of some brassinosteroid responsive-genes. Works with BRAVO to regulate QC division in the root.
UniProt Code:	Q9LN63
NCBI GenInfo Identifier:	57012618
NCBI Gene ID:	838518
NCBI Accession:	Q9LN63.1
UniProt Secondary Accession:	Q9LN63,Q8LCX8, Q94JU1, Q9SEG5,
UniProt Related Accession:	Q9LN63
Molecular Weight:	335 aa
NCBI Full Name:	Protein BRASSINAZOLE-RESISTANT 2
NCBI Official Symbol:	BES1
NCBI Official Synonym Symbols:	107 PROTEIN; BRASSINAZOLE-RESISTANT 2; BRI1-EMS-SUPPRESSOR 1; BZR2; F18O14.7; F18O14_7
NCBI Protein Information:	Brassinosteroid signaling positive regulator (BZR1) family protein
UniProt Protein Name:	Protein BRASSINAZOLE-RESISTANT 2
UniProt Synonym Protein Names:	Protein 107; Protein BIN2 SUBSTRATE 1
Protein Family:	Protein
UniProt Gene Name:	BZR2

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)