Beta Actin Monoclonal Antibody

Actins are family of highly conserved proteins that are involved in various types of cell motility, structure, and integrity. There are six different actin isoforms found in humans. These are encoded by 6 different genes: α-skeletal (expressed predominantly in striated muscle cells), α-cardiac (expressed predominantly in striated muscle cells), α-smooth (expressed predominantly in smooth muscle cells), γ-smooth (expressed predominantly in smooth muscle cells), and two ubiquitously expressed cytoplasmic (non-muscle) isoforms, β-cytoplasmic and γ-cytoplasmic. More generally, actins can be classified into 3 subgroups: alpha, beta, and gamma actins. It has been discovered that alpha actins are a major constituent of the contractile apparatus in muscle cells. Beta and gamma actins on the other hand primarily function in internal cell motility as components of the cytoskeleton.

Actin participates in many important cellular processes, including cell migration, cell division and cytokinesis, vesicle and organelle movement, cell signalling and the regulation of gene expression. Actins also help to establish and maintain cell shape and cell junctions.

The most common application Beta Actin is its use as a loading control in Western Blot for data normalization. Normalization of Western Blot data is an analytical step that is performed to compare the relative abundance of a specific protein across the lanes of a blot or gel under diverse experimental treatments, or across tissues or developmental stages.

The reason why this is carried out in Western Blot analysis is to control for experimental variation which introduced either via human error (e.g. inconsistent sample preparation, unequal sample loading across gel lanes, uneven protein transfer).

Beta Actin is one of the most common housekeeping genes used in Western Blot experiments. This is because they are thought to be expressed constitutively, at the same levels, across experiments. In this way, it can be used an an internal control to ensure experimental results are accurate and reproducible.

Our Beta Actin (ACTB) Monoclonal Antibody (CABC026) is one of the most sensitive on the market and allows researchers to dilute the antibody 1:2,560,000 for detection by Western Blot.

At those dilution factors you'll never need to buy another Beta Actin antibody.This saves time and money, and means the focus can be on the science!

It may also be used for Immunohistochemistry, and Immunofluorescence experiments and has a wide species reactivity (Human, Mouse, Rat, Chicken, Zebrafish, Pig, Oryza sativa).

To buy our Beta Actin monoclonal antibody click here.