Beta-Hydroxybutyrate (Beta-HB) Colorimetric Assay Kit (BN00856)

(No reviews yet) Write a Review
SKU:
BN00856
€619

Description

ELISA Kit Technical ManualMSDS

Beta-Hydroxybutyrate (Beta-HB) Colorimetric Assay Kit

Diabetic ketoacidosis occurs when circulating insulin levels drop to very low levels, shutting off the supply of glucose to the body. The physiological response is for the liver to produce ketone bodies (acetoacetate, acetone, and primarily Beta-hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very high rate of ketone body production outstrips the body's ability to utilize them as an energy source and the blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH. Assay Genie's Beta-HB Assay kit utilizes Beta-HB Dehydrogenase to generate a product which reacts with our colorimetric probe with an absorbance band at 450 nm. The kit is an easy and accurate assay to measure Beta-HB levels in biological samples. The assay is linear for 1-20 nmol Beta-HB in up to 100 µl samples or 0.01-0.2 mM of Beta-HB samples.

Figure 1: β-HB Standard Curve: Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00856

Size

100 assays

Detection Method

Absorbance (450 nm)

Species Reactivity

Mammalian

Applications

The assay is linear for 1-20 nmol Beta-HB in up to 100 µl samples or 0.01-0.2 mM of Beta-HB samples.

Features and Benefits

  • Simple procedure; takes ~ 40 minutes
  • Fast and convenient
  • The kit provides an accurate assay for measuring Beta-Hydroxybutyrate (Beta-HB) in various sample

Kit Components

  • Beta-HB Assay Buffer
  • Beta-HB Enzyme Mix
  • Beta-HB Substrate Mix
  • Beta-HB Standard (1.0 µmol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose