Beta-Lactamase Activity Colorimetric Assay Kit (BN01019)

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BN01019
  • Beta-Lactamase Activity Colorimetric Assay Kit (BN01019)
  • Beta-Lactamase Activity Colorimetric Assay Kit (BN01019)
  • Beta-Lactamase Activity Colorimetric Assay Kit (BN01019)
€888

Description

ELISA Kit Technical ManualMSDS

Beta-Lactamase Activity Colorimetric Assay Kit

Beta-Lactamases (BetaLs), are a large family of hydrolases comprising more than 850 identified members expressed in Gram-positive and Gram-negative bacteria. BetaLs can be classified according to their substrate or inhibitor specificity. These enzymes are capable of hydrolyzing four atom rings known as Beta-lactams. Antibiotics containing Beta-lactam rings (i.e. penicillin, cephalosporin, monobactam, carbapenem) are highly susceptible to be hydrolyzed via enzymatic activity, which deactivates their antibiotic potency. BetaLs have become a significant clinical threat due to the alarming number of cases of bacterial strains showing Beta-lactam antibiotic resistance. Assay Genie's Beta-Lactamase Activity Assay Kit offers a simple and sensitive assay that can detect and quantify the enzymatic activity of these hydrolases. The assay is based on the hydrolysis of Nitrocefin, a chromogenic cephalosporin, that results in the generation of a colored product (OD 490 nm), which is directly proportional to the amount of BetaL activity. The assay can detect enzymatic activity as low as 0.06 mU in a variety of biological samples.

Figure: a) Nitrocefin Standard Curve. b) βL activity in E. coli culture (5 μl), contaminated media (CM; 30 μl) & Positive Control (4 μl). c) βL Activity of E.Coli and contaminated media expressed per milligram of protein. Assay was performed following the kit protocol.

Key Information Description

Product SKU

BN01019

Size

100 assays

Detection Method

Absorbance (490 nm)

Applications

  • Measurement of Beta-Lactamase activity in various biological samples
  • Analysis of Beta-Lactamase activity in pathological conditions

Features and Benefits

  • Rapid, simple & sensitive
  • The assay can detect enzymatic activity as low as 0.06 mU in a variety of biological samples.

Kit Components

  • BetaL Assay Buffer
  • Nitrocefin (in DMSO)
  • Positive Control (Lyophilized)
  • BetaL Hydrolysis Buffer

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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