Anti-BRD4 Antibody (CAB2249)
- SKU:
- CAB2249
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-BRD4 Antibody |
| Antibody SKU: | CAB2249 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human BRD4 (NP_490597.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, HeLa, NIH/3T3 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human BRD4 (NP_490597.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 23476 |
| Uniprot: | O60885 |
| Cellular Location: | Chromosome, Nucleus |
| Calculated MW: | 80kDa/88kDa/152kDa |
| Observed MW: | 200kDa |
| Synonyms: | BRD4, CAP, HUNK1, HUNKI, MCAP |
| Background: | The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. |
| UniProt Code: | O60885 |
| NCBI GenInfo Identifier: | 7657218 |
| NCBI Gene ID: | 23476 |
| NCBI Accession: | NP_055114.1 |
| UniProt Secondary Accession: | O60885,O60433, Q4G0X8, Q86YS8, Q96PD3, |
| UniProt Related Accession: | O60885 |
| Molecular Weight: | 152kDa |
| NCBI Full Name: | bromodomain-containing protein 4 isoform short |
| NCBI Synonym Full Names: | bromodomain containing 4 |
| NCBI Official Symbol: | BRD4Â Â |
| NCBI Official Synonym Symbols: | CAP; MCAP; HUNK1; HUNKIÂ Â |
| NCBI Protein Information: | bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein |
| UniProt Protein Name: | Bromodomain-containing protein 4 |
| UniProt Synonym Protein Names: | Protein HUNK1 |
| Protein Family: | Bromodomain-containing protein |
| UniProt Gene Name: | BRD4Â Â |
| UniProt Entry Name: | BRD4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BRD4 antibody (CAB2249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat testis using BRD4 antibody (CAB2249) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using BRD4 antibody (CAB2249) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophageal using BRD4 antibody (CAB2249) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-2 OS cells using BRD4 Polyclonal Antibody (CAB2249) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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