Anti-C3/C3a Antibody (CAB13283)

Antibody Name:	Anti-C3 / C3a Antibody
Antibody SKU:	CAB13283
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1364-1663 of human C3 / C3a (NP_000055.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, HL-60, HeLa, THP-1, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1364-1663 of human C3 / C3a (NP_000055.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KVTI KPAP ETEK RPQD AKNT MILE ICTR YRGD QDAT MSIL DISM MTGF APDT DDLK QLAN GVDR YISK YELD KAFS DRNT LIIY LDKV SHSE DDCL AFKV HQYF NVEL IQPG AVKV YAYY NLEE SCTR FYHP EKED GKLN KLCR DELC RCAE ENCF IQKS DDKV TLEE RLDK ACEP GVDY VYKT RLVK VQLS NDFD EYIM AIEQ TIKS GSDE VQVG QQRT FISP IKCR EALK LEEK KHYL MWGL SSDF WGEK PNLS YIIG KDTW VEHW PEED ECQD EENQ KQCQ DLGA FTES MVVF GCPN
Gene ID:	718
Uniprot:	P01024
Cellular Location:	Secreted
Calculated MW:	187kDa
Observed MW:	120kDa/180kDa

Synonyms:

C3, AHUS5, ARMD9, ASP, C3a, C3b, CPAMD1, HEL-S-62p, complement C3, C3 / C3a

Background:

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.

UniProt Protein Function:	C3: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Defects in C3 are the cause of complement component 3 deficiency (C3D). A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis. Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin- containing structure known as Bruch membrane. Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Inhibitor; Secreted Chromosomal Location of Human Ortholog: 19p13.3-p13.2 Cellular Component: extracellular space; extracellular region; plasma membrane Molecular Function:protein binding; endopeptidase inhibitor activity; C5L2 anaphylatoxin chemotactic receptor binding; receptor binding Biological Process: regulation of immune response; complement activation, alternative pathway; signal transduction; fatty acid metabolic process; complement activation; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; positive regulation of activation of membrane attack complex; positive regulation of type IIa hypersensitivity; positive regulation of G-protein coupled receptor protein signaling pathway; regulation of complement activation; innate immune response; immune response; positive regulation of protein amino acid phosphorylation; inflammatory response; complement activation, classical pathway Disease: Complement Component 3 Deficiency, Autosomal Recessive; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 5; Macular Degeneration, Age-related, 9
NCBI Summary:	Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. A peptide (C3a) derived from the encoded protein has antimicrobial activity, so people with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq, Nov 2014]
UniProt Code:	P01024
NCBI GenInfo Identifier:	119370332
NCBI Gene ID:	718
NCBI Accession:	P01024.2
UniProt Secondary Accession:	P01024,A7E236,
UniProt Related Accession:	P01024
Molecular Weight:
NCBI Full Name:	Complement C3
NCBI Synonym Full Names:	complement component 3
NCBI Official Symbol:	C3
NCBI Official Synonym Symbols:	ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
NCBI Protein Information:	complement C3; prepro-C3; C3a anaphylatoxin; complement component C3; complement component C3a; complement component C3b; acylation-stimulating protein cleavage product; epididymis secretory sperm binding protein Li 62p; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
UniProt Protein Name:	Complement C3
UniProt Synonym Protein Names:	C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1Cleaved into the following 12 chains:Complement C3 beta chain; C3-beta-c; C3bc; Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein; ASPAlternative name(s):C3adesArg
Protein Family:	Complement C3
UniProt Gene Name:	C3
UniProt Entry Name:	CO3_HUMAN

	Western blot analysis of extracts of various cell lines, using C3 / C3a antibody (CAB13283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts of various cell lines, using C3 / C3a Polyclonal Antibody (CAB13283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human prostate using C3 / C3a antibody (CAB13283) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of rat liver using C3 / C3a Rabbit pAb (CAB13283) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of human liver cancer using C3 / C3a Rabbit pAb (CAB13283) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-C3 / C3a Antibody (CAB6879)	Human Complement C3 / C3 ELISA Kit
Anti-C3 Antibody (CAB11196)	Human C3 (Complement Component 3) CLIA Kit (HUES00501)
Anti-C3 Antibody (CAB16781)	Human C3a (Complement Component 3a) ELISA Kit (HUES01954)
	Human C3b (Complement Fragment 3b) ELISA Kit (HUES01956)
	Human C3c (Complement Fragment 3c) ELISA Kit (HUES01957)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)