Anti-CAMLG Antibody (CAB13720)

Antibody Name:	Anti-CAMLG Antibody
Antibody SKU:	CAB13720
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human CAMLG (NP_001736.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HepG2, HT-29, Mouse thymus, Mouse testis, Rat brain, Rat thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human CAMLG (NP_001736.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MESM AVAT DGGE RPGV PAGS GLSA SQRR AELR RRKL LMNS EQRI NRIM GFHR PGSG AEEE SQTK SKQQ DSDK LNSL SVPS VSKR VVLG DSVS TGTT DQQG GVAE VKGT QLGD KLDS FIKP PECS SDVN LELR QRNR GDLT ADSV QRGS RHGL EQYL SRFE EAMK LRKQ LISE KPSQ EDGN TTEE FDSF RI
Gene ID:	819
Uniprot:	P49069
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	32kDa
Observed MW:	38kDa

Synonyms:

CAMLG, CAML, GET2

Background:

The immunosuppressant drug cyclosporin A blocks a calcium-dependent signal from the T-cell receptor (TCR) that normally leads to T-cell activation. When bound to cyclophilin B, cyclosporin A binds and inactivates the key signaling intermediate calcineurin. The protein encoded by this gene functions similarly to cyclosporin A, binding to cyclophilin B and acting downstream of the TCR and upstream of calcineurin by causing an influx of calcium. This integral membrane protein appears to be a new participant in the calcium signal transduction pathway, implicating cyclophilin B in calcium signaling, even in the absence of cyclosporin.

UniProt Protein Function:	CAMLG: Likely involved in the mobilization of calcium as a result of the TCR/CD3 complex interaction. Binds to cyclophilin B.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass; Endoplasmic reticulum Chromosomal Location of Human Ortholog: 5q23 Cellular Component: membrane; endoplasmic reticulum; integral to membrane Molecular Function:protein binding; cell adhesion molecule binding Biological Process: receptor recycling; epidermal growth factor receptor signaling pathway; viral reproduction; defense response; signal transduction
NCBI Summary:	The immunosuppressant drug cyclosporin A blocks a calcium-dependent signal from the T-cell receptor (TCR) that normally leads to T-cell activation. When bound to cyclophilin B, cyclosporin A binds and inactivates the key signaling intermediate calcineurin. The protein encoded by this gene functions similarly to cyclosporin A, binding to cyclophilin B and acting downstream of the TCR and upstream of calcineurin by causing an influx of calcium. This integral membrane protein appears to be a new participant in the calcium signal transduction pathway, implicating cyclophilin B in calcium signaling, even in the absence of cyclosporin. [provided by RefSeq, Jul 2008]
UniProt Code:	P49069
NCBI GenInfo Identifier:	4502559
NCBI Gene ID:	819
NCBI Accession:	NP_001736.1
UniProt Secondary Accession:	P49069,P49070,
UniProt Related Accession:	P49069
Molecular Weight:	32,953 Da
NCBI Full Name:	calcium signal-modulating cyclophilin ligand
NCBI Synonym Full Names:	calcium modulating ligand
NCBI Official Symbol:	CAMLG
NCBI Official Synonym Symbols:	CAML
NCBI Protein Information:	calcium signal-modulating cyclophilin ligand; calcium-modulating cyclophilin ligand; calcium-signal modulating cyclophilin ligand; cyclophilin B-binding protein
UniProt Protein Name:	Calcium signal-modulating cyclophilin ligand
UniProt Gene Name:	CAMLG
UniProt Entry Name:	CAMLG_HUMAN

	Western blot analysis of extracts of various cell lines, using CAMLG antibody (CAB13720) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human stomach using CAMLG antibody (CAB13720) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using CAMLG antibody (CAB13720) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH-3T3 cells using CAMLG Polyclonal Antibody (CAB13720) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using CAMLG Polyclonal Antibody (CAB13720) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)