Anti-CARD6 Antibody (PACO62607)

Antibody Name:	CARD6 Antibody (PACO62607)
Antibody SKU:	PACO62607
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Caspase recruitment domain-containing protein 6 protein (666-930AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO62607 diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of U251 cells with PACO62607 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:	May be involved in apoptosis.
Synonyms:	Caspase recruitment domain-containing protein 6, CARD6

UniProt Protein Function:	CARD6: May be involved in apoptosis.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 5p13.1
NCBI Summary:	This gene encodes a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9BX69
NCBI GenInfo Identifier:	209572595
NCBI Gene ID:	84674
NCBI Accession:	Q9BX69.2
UniProt Secondary Accession:	Q9BX69,Q52LR2,
UniProt Related Accession:	Q9BX69
Molecular Weight:	116,468 Da
NCBI Full Name:	Caspase recruitment domain-containing protein 6
NCBI Synonym Full Names:	caspase recruitment domain family member 6
NCBI Official Symbol:	CARD6
NCBI Official Synonym Symbols:	CINCIN1
NCBI Protein Information:	caspase recruitment domain-containing protein 6
UniProt Protein Name:	Caspase recruitment domain-containing protein 6
Protein Family:	Caspase recruitment domain-containing protein
UniProt Gene Name:	CARD6

Antibodies	ELISA Kits
Anti-CARD6 Antibody (CAB13806)	CARD6 Colorimetric Cell-Based ELISA Kit
CARD6 Antibody (PACO00511)
CARD6 Antibody (PACO08185)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)