Caspase-3 Fluorometric Assay Kit (BN00013)

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SKU:
BN00013
€290

Description

Caspase-3 Fluorometric Assay Kit

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (ax = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (ax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity.

Key Information Description

Detection Method

Fluorecence (400 nm excitation filter and 505 nm emission filter)

Size Options

  • 25 Assays
  • 100 Assays
  • 200 Assays
  • 400 Assays

Species Reactivity

Mammalian

Applications

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits

  • Simple one-step procedure; takes 1-2 hours
  • Fast and convenient
  • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity

Kit Components

  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • DEVD-AFC (1 mM)
  • DTT (1 M)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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