Caspase-4 Fluorometric Assay Kit (BN00131)

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ELISA Kit Technical ManualMSDS

Caspase-4 Fluorometric Assay Kit

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (400 nm); upon cleavage of the substrate by caspase-4 or related caspases, free AFC emits a yellow-green fluorescence (505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.

Key Information Description

Detection Method

Fluorescence (Ex/Em 400/505 nm)

Size Options

  • 25 Assays
  • 100 Assays
  • 200 Assays
  • 400 Assays

Species Reactivity



Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits

  • Simple one-step procedure; takes only 1-2 hours
  • Fast and convenient
  • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity.

Kit Components

  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • LEVD-AFC (1 mM)
  • DTT (1 M)

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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