Cathepsin E Inhibitor Screening Kit (Fluorometric) (BN00446)

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SKU:
BN00446
€1,004

Description

ELISA Kit Technical ManualMSDS

Cathepsin E Inhibitor Screening Kit (Fluorometric)

Cathepsin E (CTSE, EC: 3.4.23.34) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of the gastric cancers. Assay Genie's Cathepsin E Inhibitor Screening Kit utilizes the ability of Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin E-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the MCA fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize the potential inhibitors of Cathepsin E.

Figure: Inhibition of Cathepsin E activity by CTSE Inhibitor. Assay was performed following the kit protocol.

Key Information Description

Product SKU

BN00446

SIZE

100 assays

Detection Method

Fluorescence (Ex/Em = 320/420 nm)

Application

Screen/study/characterize potential inhibitors of Cathepsin E

Features and Benefits

  • Convenient & Rapid
  • High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin E
  • Cathepsin E inhibitor is included in the kit

Kit Components

  • CTSE Assay Buffer
  • Human Cathepsin E
  • CTSE Substrate
  • CTSE Inhibitor (1 mM Pepstatin A in DMSO)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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